| α-芋螺毒素AnIB[A11G]荧光标记的合成及应用 |
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| 引用本文： | 赵重甲,于正,刘珠果,王于,戴秋云.α-芋螺毒素AnIB[A11G]荧光标记的合成及应用[J].生物技术通讯,2010,21(4):547-550. |
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| 作者姓名： | 赵重甲 于正 刘珠果 王于 戴秋云 |
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| 作者单位： | 军事医学科学院,生物工程研究所,北京,100071 |
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| 基金项目： | 国家自然科学基金，国家岛技术研究发展计划，国家重点基础研究发展计划 |
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| 摘 要： | 目的:合成α-芋螺毒素AnIBA11G]荧光标记物,并用于测定烟碱型乙酰胆碱受体(nAChR)α3β2在小鼠脑内的分布。方法:利用两步氧化法设计合成α3β2高选择性抑制剂AnIB突变体AnIBA11G],纯化后,用5-羧基四甲基罗丹明琥珀酰亚胺酯(5-TAMRA-SE)标记,标记物鞘内注射到小鼠脑内,用激光扫描共聚焦显微镜测定α3β2在小鼠脑内的分布。结果与结论:获得了单一5-TAMRA-AnIBA11G]荧光标记物,测定表明小鼠脑内nAChRα3β2主要分布于海马区域。
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| 关 键 词： | α-芋螺毒素 AnIB[A11G] 5-羧基四甲基罗丹明琥珀酰亚胺酯 荧光标记 烟碱型乙酰胆碱受体α3β2亚型 |
Synthesis and Application of Fluorescent Analogs of α-Conotoxin AnIB[A11G] |
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| ZHAO Chong-Jia,YU Zheng,LIU Zhu-Guo,Wang Yu,DAI Qiu-yun.Synthesis and Application of Fluorescent Analogs of α-Conotoxin AnIB[A11G][J].Letters in Biotechnology,2010,21(4):547-550. |
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| Authors: | ZHAO Chong-Jia YU Zheng LIU Zhu-Guo Wang Yu DAI Qiu-yun |
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| Institution: | (Beijing Institute of Bioteehnology, Beijing 100071, China) |
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| Abstract: | Objective： To synthesize fluorescent analogs of α-conotoxin AnlB AllG] and determine the distribution of nAChR α3β2 in mouse brain. Methods： AnlBAllG], which target nAChR α3β2 subtype, was synthesized by the two-step oxidation and labeled with 5-TAMRA-SE in the the N-terminus. After purification by HPLC, 5-TAMRA-AnlBAllG] was injected to the mouse brain and the distribution of nAChR α3β2 was determined with confocol laser scanning microscopy. Results ＆ Conclusion： The pure 5-TAMRA-SE fluorescent analog of α-eonotoxin AnlB AllG] was synthesized and the nAChR α3β2 in mice brain is mainly distributed on hip- pocampus. |
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| Keywords: | AnIB[A11G] |
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