| 小鼠脂联素受体2基因编码区的克隆及序列分析 |
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| 引用本文： | 王会中,陈竞新,詹林盛,王全立.小鼠脂联素受体2基因编码区的克隆及序列分析[J].生物技术通讯,2005,16(1):9-12. |
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| 作者姓名： | 王会中 陈竞新 詹林盛 王全立 |
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| 作者单位： | 军事医学科学院,输血研究所,北京,100850 |
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| 摘 要： | 构建小鼠脂联素受体2(mAdipoR2)基因cDNA克隆，并进行序列及基因结构分析，为进一步研究小鼠AdipoR2的表达和生物学活性奠定基础。用RT—PCR方法扩增mAdipoR2基因cDNA，获得的片段连接至pGEM-T载体，转化JM109大肠杆菌，经酶切鉴定后，进行序列测定。利用因特网生物信息学资源分析mAdipoR2基因序列。结果成功地构建了mAdipoR2基因cDNA克隆，其序列与GenBank登录序列一致。通过与小鼠基因组序列比对，分析了mAdipoR2基因结构，其编码序列由7个外显子组成，编码1个含7个跨膜区的膜蛋白，但该受体不属于G蛋白偶联受体家族(GPCRs)。mAdipoR2与人及大鼠蛋白的同源性分别为91％和95％。
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| 关 键 词： | 小鼠 脂联素 受体2 基因克隆 序列分析 基因结构 RT—PCR 蛋白类激素 |
| 文章编号： | 1009-0002(2005)01-0009-04 |
Cloning and Sequencing of Mouse Adiponectin Receptor 2 Gene Encoding Region |
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| WANG Hui-zhong,CHEN Jing-xin,ZHAN Lin-sheng,WANG Quan-liInstitute of Transfusion Medicine,Academy of Military Medical Sciences,Beijing ,China.Cloning and Sequencing of Mouse Adiponectin Receptor 2 Gene Encoding Region[J].Letters in Biotechnology,2005,16(1):9-12. |
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| Authors: | WANG Hui-zhong CHEN Jing-xin ZHAN Lin-sheng WANG Quan-liInstitute of Transfusion Medicine Academy of Military Medical Sciences Beijing China |
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| Institution: | WANG Hui-zhong,CHEN Jing-xin,ZHAN Lin-sheng,WANG Quan-liInstitute of Transfusion Medicine,Academy of Military Medical Sciences,Beijing 100850,China |
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| Abstract: | To construct the cDNA clone and analyze the sequence of mouse adiponectin receptor 2 gene (mAdipoR2), and make a base for further research on expression and biological activities. The full mouse adipoR2 cDNA fragment was amplified by RT-PCR from mouse liver. The obtained fragment was cloned into pGEM-T vector, transformed to E.coli JM109 and identified by endonuclease digestion and sequencing. The cloned mAdipoR2 cDNA sequence was same as the reported on GenBank. mAdipoR2 protein share 91% and 95% identity with human's and rat's, respectively. |
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| Keywords: | adiponectin receptor 2 gene cloning sequencing |
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