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| 褪黑素通过提高p53和Fas的表达促进小鼠肝癌H22细胞凋亡 | |
| 引用本文： | 佘美华,黄春林,陈蓓蓓,王西明.褪黑素通过提高p53和Fas的表达促进小鼠肝癌H22细胞凋亡[J].生物技术通讯,2009,20(1):28-30. |
| 作者姓名： | 佘美华 黄春林 陈蓓蓓 王西明 |
| 作者单位： | 1. 南华大学,生化教研室,湖南,衡阳,421001;华中科技大学,同济医学院生化教研室,湖北,武汉,430030 2. 南华大学,生化教研室,湖南,衡阳,421001 3. 华中科技大学,同济医学院生化教研室,湖北,武汉,430030 |
| 摘 要： | 目的：研究褪黑素（MLT）对小鼠肝癌细胞株H22的促凋亡作用及其机理。方法：采用丫啶橙（AO）染色、培养液乳酸脱氢酶（LDH）活性检测和流式细胞术（FCM）观察MLT的促凋亡作用；采用RT-PCR方法检测MLT处理前后细胞的p53 mRNA、Fas mRNA的水平。结果：AO染色后H22细胞呈现明显核浓缩的凋亡形态；培养液LDH活性检测及FCM分析均提示MLT诱导H22细胞发生凋亡；RT-PCR结果显示p53、Fas表达增强。结论：MLT能促进H22细胞p53和Fas的表达，从而诱导细胞发生凋亡。 |
| 关 键 词： | 褪黑素 H22细胞株 凋亡 p53 Fas |
Melatonin Induced Apoptosis of H22 Cells by Increasing Expression of p53 and fas |
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| SHE Mei-Hua,HUANG Chun-Lin,CHEN Bei-Bei,WANG Xi-Ming.Melatonin Induced Apoptosis of H22 Cells by Increasing Expression of p53 and fas[J].Letters in Biotechnology,2009,20(1):28-30. | |
| Authors: | SHE Mei-Hua HUANG Chun-Lin CHEN Bei-Bei WANG Xi-Ming |
| Institution: | 1.Department of Biochemistry and Molecular Biology, School of Life Sciences and Technology, University of South China, Hengyang 421001; 2. Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Sciece and Technology, Wuhan 430030, China) |
| Abstract: | Objective： To investigate the mechanism of apoptosis effect on H22 hepatoma cells induced by melatonin （MLT）. Methods： Apoptosis effect of MLT on H22 cells was studied by acridine orange（AO） staining, lactate dehydrogenase（LDH） assay and flow cytometry（FCM）. Comparing the levels of p53 mRNA and Fas mRNA between cells treated with and without MLT by RT-PCR. Results： The nuclear shrinking and fragments were obvious after AO staining. LDH activity increased after MLT treatment, and a higher rate of apoptosis was observed by FCM. The expressions of p53 and Fas gene was stimulated by MLT（P〈0.01）. Conclusion： MLT induced apotosis of the H22 cells dependent on p53 and Fas. |
| Keywords: | p53 Fas |
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