营养动脉结扎和液氮冷冻对股骨头内局部氧分压的影响

目的：观察营养动脉结扎和液氮冷冻对股骨头内局部氧分压的影响，为后期制作极度低氧股骨头缺血坏死模型打下基础。方法：9只雄性大耳白兔随机平均分为对照组、液氮冷冻组和动脉结扎＋液氮冷冻组，对照组动物不做任何处理，液氮冷冻组动物行股骨头液氮冷冻使其发生缺血坏死，动脉结扎＋液氮冷冻组动物先结扎旋股内外侧动脉，再对股骨头进行液氮冷冻。造模后立刻活体观察股骨头内氧分压变化。结果：液氮冷冻组股骨头内氧分压较对照组下降一半左右，2组比较有显著性差异（P〈0．05）；动脉结扎＋液氮冷冻组动物股骨头内氧分压进一步下降到对照组的1／8左右，与其他2组相比有显著性差异（P〈0．05）。结论：液氮冷冻和旋股内、外侧动脉结扎均可明显降低股骨头内氧分压，两者合用具有重叠效应，可共同促使股骨头内氧分压下降。

Influence of Oxygen Partial Pressure of Femoral Head After Nour-ishing Blood Ligation and Liquid Nitrogen Frozen

Objective： To observe the influence of nutrient artery ligation and liquid nitrogen frozen on partial pressure of oxygen in the femoral head, and to explore the possibility of making avascular necrosis of femoral head （ANFH） model via extremely hypoxia. Methods： 9 male white rabbits were randomly divided into control group, liquid nitrogen frozen group, nutrient artery ligation and liquid nitrogen frozen group on average. Control group re- ceived no treatment, liquid nitrogen frozen group was established the models of ANFH by liquid nitrogen frozen method, nutrient artery ligation and liquid nitrogen frozen group was established the models of ANFH by ligating nutrient artery of femoral head before liquid nitrogen frozen. The self-made complex transducer of partial oxygen pressure and intelligent four-channel partial oxygen pressure detector were adopted to monitor the change of par-tial oxygen pressure in femoral head. Results： The partial pressure of oxygen in the femoral head of the liquid ni-trogen frozen group declined by half compared with control group, there are significant differences between two groups（P〈0.05）. The partial pressure of oxygen in the femoral head of the nutrient artery ligation and liquid nitro-gen frozen group further declined to one-eighth of control group, there are significant differences compared with other groups（P〈0.05）. Conclusion： Nutrient artery ligation and liquid nitrogen frozen could significantly reduced partial pressure of oxygen in the femoral head, and combination of two methods would further decline the partial pressure of oxygen in the femoral head.