雷公藤甲素诱导胰腺癌细胞凋亡

目的：观察雷公藤甲素对胰腺癌细胞BxPC-3和PANC-1生长抑制及诱导细胞凋亡作用，探讨雷公藤甲素抗胰腺癌的机制。方法：雷公藤甲素处理BxPC-3和PANC—1细胞后，用M1rr法检测细胞的生长抑制，用流式细胞术检测细胞的凋亡率，用罗丹明123和DCFH—DA染色方法测定细胞线粒体膜跨膜电位变化和活性氧（ROS）的产生，用Western印迹检测Bcl-2、Bax蛋白表达的变化。结果：雷公藤甲素对胰腺癌细胞BxPC-3和PANC—1具有生长抑制和诱导细胞凋亡的作用，且呈时间和剂量依赖关系；处理72h后，胰腺癌细胞线粒体跨膜电位明显下降，Bax表达上调，Bcl-2表达下降。结论：雷公藤甲素能有效抑制胰腺癌细胞增殖，通过增强线粒体通透性诱导细胞凋亡。

Triptolide Induces Apoptosis of Human Pancreatic Cancer Cells

Objective： To determine whether triptolide inhibits proliferation and induces apoptosis of pancreatic cancer cells. Methods： The effects of triptolide on the antiproliferative activity of pancreatic cancer cells BxPC-3 and PANC-I were determined by MTT assays. Apoptosis of pancreatic cancer cells induced by triptolide were de- termined by flow cytometry analysis. Mitochondrial membranere potential and ROS production analysis were detect- ed by Rhodamine 123 and DCFH-DA. The effects of triptolide on expression levels of Bcl-2 and Bax were deter- mined by Western blotting. Results： Triptolide displayed inhibition activity of cells growth and induction of apopto- sis on pancreatic cancer cells. Triptolide induced the loss of mitochondrial membrane potential by regulating the expression levels of Bcl-2 family proteins. Conclusion： The results suggest that triptolide may inhibit proliferation and induce apoptosis of pancreatic cancer cells by inducing the loss of mitochondrial membrane potential.