| 实时荧光定量PCR及其在传染性疾病检测中的应用 |
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| 引用本文： | 邹红霞,梁国鲁.实时荧光定量PCR及其在传染性疾病检测中的应用[J].生物技术通讯,2011,22(5):751-754. |
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| 作者姓名： | 邹红霞 梁国鲁 |
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| 作者单位： | 西南大学园艺园林学院,重庆40 |
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| 摘 要： | 实时荧光定量PCR技术被广泛应用于实验研究、临床检测中。与普通的PCR相比,实时荧光定量PCR技术具有特异性强、灵敏度高、重复性好、定量准确、速度快、全封闭反应等优点。我们综述了实时荧光定量PCR技术的原理、定量方法,及其在传染性疾病检测研究中的应用。
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| 关 键 词： | 实时荧光定量PCR 原理 定量方法 传染性疾病 |
Real-Time Quantitative PCR Technology and its Application in Detection of Infectious Diseases |
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| ZOU Hong-Xia,LIA NG Guo-Lu.Real-Time Quantitative PCR Technology and its Application in Detection of Infectious Diseases[J].Letters in Biotechnology,2011,22(5):751-754. |
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| Authors: | ZOU Hong-Xia LIA NG Guo-Lu |
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| Institution: | ZOU Hong-Xia,LIANG Guo-Lu Horticulture and Landscape Architecture College,Southwestern University,Chongqing 40,China |
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| Abstract: | In recent years,real-time quantitative PCR technology has been widely used in various experimental studies,clinical testing and production.Compared to normal PCR,real-time quantitative PCR technology is ad-vanced in specificity,sensitivity,reproducibility,accuracy,speed and closed.This paper is a review about the principle,quantitative method of real-time fluorescence quantitative PCR technology,and the application in detec-tion of infectious diseases. |
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| Keywords: | real-time fluorescence quantitative PCR principle quantitative methods infectious diseases |
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