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| 重组人内皮单核细胞活化多肽Ⅱ原对Saos-2细胞基因的表达调控 | |
| 引用本文： | 邹亮,王磊,刘大涛,邢玉华,王慧敏,胡立德,谭小钉,刘刚,陈惠鹏.重组人内皮单核细胞活化多肽Ⅱ原对Saos-2细胞基因的表达调控[J].生物技术通讯,2009,20(5):626-628,634. |
| 作者姓名： | 邹亮 王磊 刘大涛 邢玉华 王慧敏 胡立德 谭小钉 刘刚 陈惠鹏 |
| 作者单位： | 1. 军事医学科学院,生物工程研究所,北京100071;吉林大学,生命科学学院,吉林,长春,130012 2. 军事医学科学院,附属医院,北京100071 3. 信谊药厂,上海,201206 4. 军事医学科学院,北京100071;吉林大学,生命科学学院,吉林,长春,130012 5. 军事医学科学院,北京100071;安徽大学,生命科学学院,安徽,合肥,230039 6. 军事医学科学院,生物工程研究所,北京100071 |
| 摘 要： | 目的：将重组人内皮单核细胞活化多肽Ⅱ原（pro-EMAPⅡ）作用于Saos-2细胞,研究后者相关基因表达水平的变化。方法：在大肠杆菌中表达重组人pro-EMAPⅡ,并纯化获得目的蛋白;利用基因芯片技术研究重组人pro-EMAPⅡ对Saos-2细胞基因的表达调控。结果：获得重组人pro-EMAPⅡ;基因表达芯片分析表明,重组人pro-EMAPⅡ作用于Saos-2细胞后,后者有39个基因表达上调,29个基因表达下调,在这68个基因中有12个差异基因参与多个信号通路。结论：重组人pro-EMAPⅡ作用于Saos-2细胞,使其多个基因表达水平发生改变,有助于揭示pro-EMAPⅡ的调控机制及信号通路。 |
| 关 键 词： | 重组人内皮单核细胞活化多肽Ⅱ原 Saos-2细胞 基因芯片 表达调控 |
Regulation of Gene Expression in Saos-2 Cells Induced by Recombinant Human pro-Endothelial Monocyte-Activating Polypeptide Ⅱ |
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| ZOU Liang,WANG Lei,LIU Da-Tao,XING Yu-Hua,WANG Hui-Min,HU Li-De,TAN Xiao-Ding,LIU Gang,CHEN Hui-Peng.Regulation of Gene Expression in Saos-2 Cells Induced by Recombinant Human pro-Endothelial Monocyte-Activating Polypeptide Ⅱ[J].Letters in Biotechnology,2009,20(5):626-628,634. | |
| Authors: | ZOU Liang WANG Lei LIU Da-Tao XING Yu-Hua WANG Hui-Min HU Li-De TAN Xiao-Ding LIU Gang CHEN Hui-Peng |
| Institution: | 1. Beijing Institute of Biotechnology, Beijing 100071; 2. Affiliated Hospital of Academy of Military Medical Sciences, Beijing 100071; 3. Sine Pharmaceu, Shanghai 201206; 4. College of Life Science, Jilin University, Changchun 130012; 5. College of Life Science, Anhui University, Hefei 230039; China) |
| Abstract: | Objective：To investigate the variation of the related gene expression in Saos-2 cells induced by recombinant human pro-endothelial monocyte-activating polypeptideⅡ（pro-EMAPⅡ）.Methods：The recombinant human pro-EMAPⅡ（rhpro-EMAPⅡ） was exprssed in Escherichia coli,and then was purified.The regulation of gene expression in Saos-2 cells induced by rhpro-EMAPⅡ was analyzed by gene chips.Results：rhpro-EMAPⅡ was received,and the analysis re-sults of gene chips revealed that there were 39 genes up-regulated and 29 genes down-regulated in Saos-2 cells. Among these genes there were 12 differentially expressed genes participating in cell signal pathway. Conclusion： rhpro-EMAPⅡ changes the levels in expression of several genes in Saos-2 cells, and it is helpful to reveal the regulation mechanism and signal pathway of pro-EMAPⅡ. |
| Keywords: | pro-endothelial monocyte-activating polypeptideⅡ Saos-2 cells gene chips expression regulation |
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