| 菲律宾蛤仔基因组DNA的提取及其RAPD分析 |
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| 引用本文： | 骆轩,江树勋,高如承,王静.菲律宾蛤仔基因组DNA的提取及其RAPD分析[J].生物技术通讯,2003,14(5):361-363. |
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| 作者姓名： | 骆轩 江树勋 高如承 王静 |
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| 作者单位： | 1. 福建师范大学,生物工程学院,福州,350007
2. 福建省出入境检验检疫局,技术中心,福州,350001 |
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| 基金项目： | 科技部农业科技成果转化资金项目2002650030480，福建省教育厅开发研究项目JA01022 |
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| 摘 要： | 对菲律宾蛤仔(Ruditapes philippinarum)的三种组织，即性腺、斧足和内脏团的基因组DNA提取方法进行了研究，并对所提取的DNA作RAPD分析。结果表明性腺的DNA的得率明显比斧足和内脏团高。并且探讨了用于对基因组DNA进行RAPD研究的4种引物及最佳PCR条件，得到了较好的RAPD结果。
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| 关 键 词： | 菲律宾蛤仔 DNA提取 随机扩增多态性DNA RAPD分析 性腺 斧足 内脏团 |
| 文章编号： | 1009-0002(2003)05-0361-03 |
| 修稿时间： | 2003年4月10日 |
The research of the genomic DNA isolation and RAPD analysison Ruditapes philippinarum |
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| LUO Xuan ,JIANG Shu-xun ,GAO Ru-cheng ,WANG Jing.The research of the genomic DNA isolation and RAPD analysison Ruditapes philippinarum[J].Letters in Biotechnology,2003,14(5):361-363. |
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| Authors: | LUO Xuan JIANG Shu-xun GAO Ru-cheng WANG Jing |
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| Institution: | LUO Xuan 1,JIANG Shu-xun 2,GAO Ru-cheng 1,WANG Jing 1 |
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| Abstract: | The methods of genomic DNA isolation of Ruditapes philippinarum were studied.DNA samples of the gonad,pelecypodium and visceral mass of R.philippinarum were extracted by such method.The results showed that the yield of genomic DNA from the gonad was higher than that from pelecypodium and visceral mass.Also four RAPD primers and the best PCR conditions was found to be able to get the well RAPD results from the gonad DNA. |
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| Keywords: | Ruditapes philippinarum DNA extraction random amplified polymorphic DNA |
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