| 重组人骨形态发生蛋白-2生物学活性测定新方法的建立 |
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| 引用本文： | 丁红梅,刘农乐,王芳,李少华,邵宁生,王会信.重组人骨形态发生蛋白-2生物学活性测定新方法的建立[J].生物技术通讯,2007,18(1):101-102. |
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| 作者姓名： | 丁红梅 刘农乐 王芳 李少华 邵宁生 王会信 |
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| 作者单位： | 军事医学科学院,基础医学研究所,北京,100850 |
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| 摘 要： | 目的:采用小鼠异位成骨技术及甲基麝香草酚蓝比色法检测重组人骨形态发生蛋白-2(rhBMP-2)的生物学活性。方法:将rhBMP-2埋入小鼠肌间隙内,14d后取出新生组织,采用血清钙试剂盒检测其钙含量。结果:随着给药组剂量递增,相应地钙含量也增加,二者具有较强的量效关系。结论:此方法为本实验室独创,较传统的血清碱性磷酸酶方法更为方便、快捷,是一种能够定量检测rhBMP-2活性的新方法。
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| 关 键 词： | 重组人骨形态发生蛋白-2 活性测定 钙含量 |
| 文章编号： | 1009-0002（2007）01-0101-02 |
| 修稿时间： | 2006-06-26 |
A New Method for Biological Activity Determination of Recombinant Human Bone Morphogenic Protein-2 |
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| DING Hong-mei,LIU Nong-le,WANG Fang,LI Shao-hua,SHAO Ning-sheng,WANG Hui-xin.A New Method for Biological Activity Determination of Recombinant Human Bone Morphogenic Protein-2[J].Letters in Biotechnology,2007,18(1):101-102. |
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| Authors: | DING Hong-mei LIU Nong-le WANG Fang LI Shao-hua SHAO Ning-sheng WANG Hui-xin |
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| Institution: | Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract: | Objective: Technique of allotopic osteogenesis in mouse and serum calcium diagnostic kit(methyl-thymol blue colormetric method) were used to determine the biological activity of recombinant human bone morphogenic protein-2(rhBMP-2). Methods: rhBMP-2 in powder form was embedded in intermuscular spatium of posterior limb. 14 days after being embeded, neoplasm in operation sites was removed and ground, and calcium content in neoplasm was measured with serum calcium diagnostic kit. Results: It showed that the calcium level increased in parallel to that of implanted rhBMP-2 in dose-dependent manner. Conclusion: A new method for rhBMP-2 activity determination is established, which is more feasible and sensitive than traditional alkaline phospatase(AKP) method. |
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| Keywords: | recombinant human bone morphogenic protein-2 activity detection calcium content |
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