| 枯草芽孢杆菌产胞苷的初步研究 |
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| 引用本文： | 黄艳辉,魏志强,徐庆阳,陈宁.枯草芽孢杆菌产胞苷的初步研究[J].生物技术通讯,2007,18(2):255-257. |
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| 作者姓名： | 黄艳辉 魏志强 徐庆阳 陈宁 |
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| 作者单位： | 1. 天津科技大学,生物工程学院,天津,300457
2. 天津科技大学,生物工程学院,天津,300457;天津市工业微生物重点实验室,天津,300457 |
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| 摘 要： | 目的:通过对野生型枯草芽孢杆菌NO122的诱变筛选,选育出胞苷产生菌株。方法:以野生型枯草芽孢杆菌为出发菌株进行紫外线、硫酸二乙酯诱变,经3-脱杂氮尿嘧啶、5-氟胞苷结构类似物平板定向筛选产胞苷突变株;研究碳源、氮源、温度、初始pH值等发酵条件对该突变菌株产胞苷的影响。结果:经诱变传代后得到突变株TZM1012,该突变株在发酵温度37℃、初始pH7.2、摇床转速220r/min的条件下,摇瓶发酵72h,发酵液中的胞苷可达1.873g/L,并具有较好的遗传稳定性。结论:获得产胞苷生产菌株,并具有较好的遗传稳定性。
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| 关 键 词： | 胞苷 枯草芽孢杆菌 发酵 育种 优化 |
| 文章编号： | 1009-0002（2007）02-0255-03 |
| 修稿时间： | 2006-09-07 |
Study on the Breeding of Cytidine-Producing Strain in Bacillus subtilis |
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| HUANG Yan-hui,WEI Zhi-qiang,XU Qing-yang,CHEN Ning.Study on the Breeding of Cytidine-Producing Strain in Bacillus subtilis[J].Letters in Biotechnology,2007,18(2):255-257. |
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| Authors: | HUANG Yan-hui WEI Zhi-qiang XU Qing-yang CHEN Ning |
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| Institution: | 1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin 300457; 2. Tianjin Key Lab of Industrial Microbiology, Tianjin 300457; China |
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| Abstract: | Objective:To derived cytidine overproducing mutant from Bacillus subtilis NO122.Methods:By means of ultraviolet and diethyl sulfate,the mutant was selected directionally and step wisely on the agar plates containing 3-deazaurcil,5-fluorocytidine.Results:The fermentation medium of TZM1012 was optimized and the fermentation condition was also studied.Under the optimal condition,the mutant could produce cytidine 1.873 g/L after 72 hours of fermentation.And also the TZM1012 strain had steady heredity.Conclusion:Cytidine overproducing mutant was obtained,the strain had steady heredity. |
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| Keywords: | cytidine Bacillus subtilis fermentation breeding optimization |
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