| 溶氧对L-缬氨酸发酵过程的影响 |
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| 引用本文： | 马雷,秦永峰,徐庆阳,谢希贤.溶氧对L-缬氨酸发酵过程的影响[J].生物技术通讯,2010,21(4):509-514. |
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| 作者姓名： | 马雷 秦永峰 徐庆阳 谢希贤 |
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| 作者单位： | 1. 天津科技大学电子信息与自动化学院,天津,300222;天津科技大学生物工程学院,天津,300457
2. 天津科技大学生物工程学院,天津,300457 |
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| 基金项目： | 国家科技重大专项，天津市应用基础及前沿技术研究计划，天津市科技支撑计划重点项目 |
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| 摘 要： | 目的:以黄色短杆菌XV0505为供试菌,探索溶氧对L-缬氨酸发酵过程的影响及其控制策略。方法:利用5L发酵罐,考察了不同溶氧浓度对L-缬氨酸发酵的影响,并采用代谢流分析对其结果进行阐述,提出分阶段溶氧控制策略。结果:采用分阶段溶氧控制策略,即在0～24h溶氧浓度为20%,24～60h溶氧浓度为5%,发酵60h,L-缬氨酸可达到58.36g/L,比5%和20%溶氧浓度下分别提高了18.95%和13.56%。结论:溶氧浓度对L-缬氨酸发酵有重要影响。
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| 关 键 词： | L-缬氨酸 溶氧 代谢流分析 发酵 |
The Effects of Dissolved Oxygen on L-Valine in Fermentation |
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| MA Lei,QIN Yong-Feng,XU Qing-Yang,XIE Xi-Xian.The Effects of Dissolved Oxygen on L-Valine in Fermentation[J].Letters in Biotechnology,2010,21(4):509-514. |
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| Authors: | MA Lei QIN Yong-Feng XU Qing-Yang XIE Xi-Xian |
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| Institution: | b a. College of Electronic Information and Automation, Tianjin 300222; b. College of Biotechnology, Tianjin 300457; Tianjin University of Science ＆ Technology, China |
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| Abstract: | Objective： To explore the effects of dissolved oxygen on the fermentation of L-valine and the strategy for contronling dissolved oxygen with Brevibacterium flavum XV0505 as production strain. Methods： The influence of dissolved oxygen on the L-valine fermentation were stutied. Then the reason was quantitatively described in detail with the distribution of flux for metabolites in metabolic network, and pose that a two-stage oxygen supply strategy. Results： On the optimum oxygen supply strategy, the productivity of L-valine was 58.36 g / L in 5 liter fermentor in 60 h, inceases 18.95% and 13.56% respectively with dissolved oxygen concent rations of 5% and 20%. Conclution： The dissolved oxygen have an important impact on the L-valine production. |
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| Keywords: | L-valine dissolved oxygen metabolic ansysis fermentation |
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