| 结核分枝杆菌脂阿拉伯甘露聚糖的提纯及活性初步鉴定 |
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| 引用本文： | 戴振华,郭兰芹,冯晓燕,张立,郄霜,杨锡琴,修冰水,陈堃,彭瑞云,张贺秋.结核分枝杆菌脂阿拉伯甘露聚糖的提纯及活性初步鉴定[J].生物技术通讯,2014(2):230-233. |
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| 作者姓名： | 戴振华 郭兰芹 冯晓燕 张立 郄霜 杨锡琴 修冰水 陈堃 彭瑞云 张贺秋 |
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| 作者单位： | [1]军事医学科学院基础医学研究所,北京100850 [2]华北石油总医院．河北任丘062552 ,北京100850 [3]天津海河医院,天津300350 [4]河北联合大学,河北唐山063000 [5]军事医学科学院放射与辐射医学研究所,北京100850 |
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| 基金项目： | “十一五”国家科技重大专项（20092X10004-718） |
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| 摘 要： | 目的:提取纯化结核分枝杆菌(MTB)脂阿拉伯甘露聚糖(LAM)。方法:MTB菌体彻底破碎后,去脂,去蛋白,上清液经苯酚萃取,酒精沉淀,得到LAM;以提取的LAM作为包被抗原检测血清中的LAM抗体。结果和结论:提取到LAM抗原,免疫印迹表明,LAM迁移范围相对分子质量为25×103~40×103,主要集中在35×103处。在64例肺结核患者中,有43例LAM-ELISA检测阳性(敏感性为67.19%);在67例健康志愿者中,有64例LAM-ELISA检测阴性(特异性为95.52%)。
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| 关 键 词： | 结核分枝杆菌 脂阿拉伯甘露聚糖 提纯 |
Purification and Identification of Mycobacterium tuberculosis Lipoara binomannan |
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| DAI Zhen-Hua,GUO Lan-Qin,FENG Xiao-Yan,ZHANG Li,QIE Shuang,YANG Xi-Qin,XIU Bing-Shui,CHEN Kun,PENG Rui-Yun,ZHANG He-Qiu.Purification and Identification of Mycobacterium tuberculosis Lipoara binomannan[J].Letters in Biotechnology,2014(2):230-233. |
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| Authors: | DAI Zhen-Hua GUO Lan-Qin FENG Xiao-Yan ZHANG Li QIE Shuang YANG Xi-Qin XIU Bing-Shui CHEN Kun PENG Rui-Yun ZHANG He-Qiu |
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| Institution: | 1. Beijing Institute of Basic Medical Sciences, Beijing 100850; 2. The General Hospital of Huabei Petroleum, Ren- qiu 062552; 3. Tianjin Haihe Hospital, Tianjin 300350; 4. Hebei United University, Tangshan 063000; 5. Beijing Institute of Radiation Medicine, Beijing 100550; China ＊Corresponding author, E-mail： zhangheqiu2004@126.com) |
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| Abstract: | Objective： To extract and purify lipoarabinomannan （LAM） from Mycobacterium tuberculosis （MTB）. Methods： The MTB was crashed. The lipids and protein were removed, the supernatant was harvested and extract ed with phenol, and precipitated with ethanol, and LAM was obtained. Results ＆ Conclusion： LAM antigen is prepared successfully. By Western blots, the obtained LAM migration was in the range 25-40 kDa, mainly concen trated in the 35 kDa place. Of the 64 tuberculosis patiens, 43 were positive using the LAM-ELISA（sensitivity 67.19%）. Of the 67 healthy volunteers, 64 were negative using the LAM-ELISA（specificity 95.52%）. |
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| Keywords: | Mycobacterium tuberculosis lipoarabinomannan purification |
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