| 抗新型隐球菌荚膜相关蛋白CAP10单克隆抗体的制备及鉴定 |
| |
| 引用本文： | 于虹,王欣,杨裔,陈万荣,郭彦,周育森,高基民,寇志华.抗新型隐球菌荚膜相关蛋白CAP10单克隆抗体的制备及鉴定[J].生物技术通讯,2014(2):203-206. |
| |
| 作者姓名： | 于虹 王欣 杨裔 陈万荣 郭彦 周育森 高基民 寇志华 |
| |
| 作者单位： | [1]军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京100071 [2]温州医科大学检验医学与生命科学学院,浙江温州325035 |
| |
| 基金项目： | 传染病防治重大专项（2008ZX10001-007） |
| |
| 摘 要： | 目的:以新型隐球菌荚膜相关蛋白CAP10为靶抗原,制备并鉴定特异性抗CAP10的单克隆抗体。方法:用纯化的重组CAP10免疫BALB/c小鼠,血清抗体效价达到适当水平时进行细胞融合;经多次亚克隆筛选出分泌特异性抗体的细胞株,制备单抗腹水并进行抗体效价测定及亚类鉴定。结果:获得11株能稳定分泌抗新型隐球菌荚膜相关蛋白CAP10的单克隆抗体杂交瘤细胞株,抗体效价高且抗原特异性强。结论:获得了针对新型隐球菌荚膜相关蛋白CAP10的单克隆抗体,为深入研究CAP10蛋白的功能,以及临床新型隐球菌的检测和血清型分析奠定了基础。
|
| 关 键 词： | 新型隐球菌 CAPIO蛋白 单克隆抗体 |
Preparation and Evaluation of Monoclonal Antibodies Specific to CAPIO Protein of Cryptococcus neoformans |
| |
| YU Hong,WANG Xin,YANG Yi,CHEN Wan-Rong,GUO Yan,ZHOU Yu-Sen,GAO Ji-Min,KOU Zhi-Hua.Preparation and Evaluation of Monoclonal Antibodies Specific to CAPIO Protein of Cryptococcus neoformans[J].Letters in Biotechnology,2014(2):203-206. |
| |
| Authors: | YU Hong WANG Xin YANG Yi CHEN Wan-Rong GUO Yan ZHOU Yu-Sen GAO Ji-Min KOU Zhi-Hua |
| |
| Institution: | 1. State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Mili tary Medical Sciences, Beijing 100071; 2. Wenzhou Medical University, Wenzhou 325035; China ＊Co-corresponding authors, KOU Zhi-I-Iua, E-mail： zh1981k06@sohu.com; GAO Ji-Min, E-mail： jimingao64@163.com) |
| |
| Abstract: | Objective： To produce monoclonal antibodies（mAb） specific to CAPIO protein of Cryptococcus neofor roans. Methods： The BALB/c mice were immunized with the recombinant CAP10-1 （70-250 aa）, and fusion of lymphocyte and myeloma Sp2/0 cell was performed by conventional method. Antibody titers and specificities of mAb were measured by ELISA followed production of mAb. Results： Eleven strains of hybridoma cell lines with the stable secretion of mAb to protein CAP10 were generated, and all the mAb only reacted with the recombinant CAP10-1. Conclusion： High titers of the specific mAb to the conserved fragment of CAPIO of C.neoformans were successfully produced, which is helpful for further study of the function of CAP10 and clinical diagnosis and sero- type analysis for infections of C.neoformans. |
| |
| Keywords: | Cryptococcus neoformans protein CAPIO monoclonal antibodies |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
