| PESl蛋白与雄激素受体的相互作用及定位 |
| |
| 引用本文： | 关鑫,王洪远,任伟,杜佩云,秘晓林,程龙,叶棋浓.PESl蛋白与雄激素受体的相互作用及定位[J].生物技术通讯,2014(3):365-368. |
| |
| 作者姓名： | 关鑫 王洪远 任伟 杜佩云 秘晓林 程龙 叶棋浓 |
| |
| 作者单位： | [1]军事医学科学院生物工程研究所,北京100850 [2]大连医科大学肿瘤干细胞研究院,辽宁大连116000 [3]安徽医科大学附属安庆市立医院口腔科,安徽安庆246600 [4]福建农林大学生命科学学院,福建福州350002 |
| |
| 基金项目： | 基金项目：北京市科技新星计划（z131102000413034） |
| |
| 摘 要： | 目的：研究PES1蛋白与雄激素受体（An）之间的相互作用。方法：利用免疫共沉淀实验检测PES1蛋白与AR之间的相互作用，并进行相互作用定位；利用Western印迹研究PESl对乳腺癌细胞内AR表达水平的影响。结果：免疫共沉淀实验显示PES1蛋白与AR存在相互作用；PES1蛋白的1—110、111-220、221-320和311-588氨基酸残基（aa）区域均能与AR结合，415～588aa不能结合AR；AR的651-918aa区域与PESl结合。PESl不能调节乳腺癌细胞AR的表达水平。结论：PES1多个区域均能与AR相互作用，并且主要结合在AR的转录激活结构域2，为进一步探讨PES1对AR功能的调节奠定了基础。
|
| 关 键 词： | PES1蛋白 雄激素受体 相互作用 乳腺癌 |
Detection and Mapping of the Interaction Between PES1 and Androgen Receptor |
| |
| GUAN Xin,WANG Hong-Yuan,REN Wei,DU Pei-Yun,BI Xiao-Lin,CHENG Long,YE Qi-Nong.Detection and Mapping of the Interaction Between PES1 and Androgen Receptor[J].Letters in Biotechnology,2014(3):365-368. |
| |
| Authors: | GUAN Xin WANG Hong-Yuan REN Wei DU Pei-Yun BI Xiao-Lin CHENG Long YE Qi-Nong |
| |
| Institution: | 1. Beijing Institute of Biotechnology, Beijing 100850; 2. Institute Dalian 116000; 3. Department of Stomatology, Affiliated Anqing Anqing 26600; 4. College of Life Science, Fujian Agriculture and of Cancer Stem Cell, Dalian Medical University, Municipal Hospital of Anhui Medical University, Forestry University, Fuzhou 350002; China) |
| |
| Abstract: | Objective： To identify the interaction of PES1 and Androgen receptor（AR）. Methods： Co-immunoprecipitation was performed to detect and map the interaction of PES1 and AR. The expression level of AR influenced by PES1 protein in breast cancer cells was investigated by Western blotting. Results： PES1 could interact with AR. PES1-110, PESI111-220, PES1221-322, and PES1311-588 interacted with AR, whereas PES1415-588 did not. On the other hand, PES1 interacted with the amino acid region 651-918 of AR. PES1 could not regulate the expression level of AR in breast cancer cells. Conclusion： Many regions of PES1 can interact with AR. PES1 interacts with the activation function 2 domain of AR. This study lays a foundation for further study on the regulation of PES1 on AR function. |
| |
| Keywords: | PES1 androgen receptor interaction breast cancer |
| 本文献已被 维普 等数据库收录！ |
|
