恶性疟原虫多抗原表位基因表达载体的构建及其在大豆幼胚中的表达

疟疾是当今最需要研究有效疫苗的主要传染病之一。AWTE基因编码恶性疟原虫多种抗原表位基因 ,CTB基因编码霍乱毒素 B亚基 ,是一种既能引起细胞免疫又能引起体液免疫的免疫载体和佐剂。把 AWTE- CTB融合基因构建到植物表达载体 p BVG- ny2上 ,通过基因枪导入法 ,转化大豆幼胚分生组织。 X- glu染色检测到 GUS基因的表达 ;抗原性分析实验结果表明 ,特异表达的融合蛋白可与 CTB和 AWTE抗体结合 ,具有 CTB抗原性。这个实验结果 ,表明疟疾多抗原表位基因首次在转基因大豆幼胚中得到瞬时表达

The expression of multiple epitopes against Plasmodium falciparum in the transformant soybean tissue

Malaria is one of the most serious pathogenesis which causes more than three million death in the world. Cholera toxin B subunit is a good carrier protein and an effective adjuvant that can boost both cellular and humoral immol/Lunity. DNA fragments encoding B cell, Th cell and CTL epitomes of Plasmodium falciparum CS, MSA 1, MSA 2 and RESA antigens were cloned down stream of cholera toxin B subunit gene in the same reading frame. Through the gene gun to transfer the AWTE CTB plant expression vector pBVG ny2 which containing NPTII and GUS gene into the soybean tissue. After selection by Kanmycine and dying with X glu to prove the CTB AWTE vector plasmid transformed into soybean tissue. Antigenicity assay showed the AWTE CTB protein expressed in the transgenic soybean tissue can combine with CTB antibody. This is the first report on Cholera Toxin B subunit and poly valent P. falciparum protective epitopes gene transient expressing in the soybean tissue.