c—Ha-ras在转基因小鼠模型C57-ras各组织中的表达谱分析

目的：分析转基因c-Ha-ras在C57-ras癌症小鼠模型中的组织表达谱及时空表达差异。方法：利用半定量及荧光定量RT-PCR法，分析不同首建鼠系、不同周龄小鼠各脏器中转基因c-Ha-ras的表达。结果：转基因c-Ha-ras在心、肝等13种组织器官中均有表达，在肺脏中表达最高，在肝脏中表达最低，No.2、No.3和No.5等3个首建鼠均呈现相似的变化规律；转基因在No.5首建鼠中表达水平最高，而在同一个首建鼠系中，12周龄时表达高于8周龄和24周龄。结论：转基因c-Ha-ras在各脏器中能高效表达，并间接表明该转基因能稳定遗传，为C57-ras癌症小鼠模型用于新药临床前致癌性评价提了供理论支持。

Gene Expression Profile Analysis on Human c-Ha-ras Gene Expression in C57-ras Cancer Transgenic Mice

Objective： To analyze gene expression profile and the time-space expression distribution of c-Ha-ras gene in C57-ras cancer transgenic mice. Methods：Primers were designed according to c-Ha-ras gene sequence, we analyzed expression profile in different founders and various tissues of C57-ras cancer transgenic mice by semi-quantitative RT-PCR and real-time RT-PCR method. Results：Analysis showed that c-Ha-ras had the high-est expressional level in lung, and lowest in liver. Similar change rule were found in No.2, No.3 and No.5 lines. In the aspect of expression in different time phase, comparison was done in the same line, 12-week C57-ras mice was higher than 8-week and 24-week. Among all the three founder lines, c-Ha-ras expressed highest in No.5. Conclusion：Transgenic c-Ha-ras expressed in the C57-ras cancer mice, which provided theoretical support for applying this mouse model in new drug preclinical carcinogenicity evaluation. Due to the highest expression in line No.5, it might be breeding and studied in carcinogenicity preferentially. Furthermore, since analysis was based on 12 generations of C57-ras mice, expression in various organs and different generations verified the stable hered-ity of c-Ha-ras gene.