猪心脏脂肪酸结合蛋白基因PCR-RFLP分子标记研究

利用PCR-RFLP分子标记技术,检测了杜洛克、长白、大白、内江、荣昌、汉江黑、汉白、八眉和野猪共计265头猪心脏脂肪酸结合蛋白基因5'上游区和第二内含子区的遗传变异。结果表明,在HinfI-RFLP位点上,上述猪种和野猪均存在多态性,等位基因H的频率分别为0.7500,0.7188,0.9167,0.3333,0.1250,0.6909,0.1167,0.8500和0.9375;除汉江黑猪(P<0.05)和野猪(P<0.01)外,其余的猪种基因频率和基因型频率都处于Hardy-Weinderg平衡状态(P>0.05);大白、八眉、汉江黑、汉白和野猪表现为低度多态(PIC<0.25),杜洛克、长白、内江和荣昌猪为中度多态性(0.25

Study on PCR-RFLP molecular marker of pig heart fatty acid-binding protein gene

The utilization of transgenic plants as bioreactor expressing recombinant protein for production of animal vaccine is a attractive and economic system. Here, a expression plasmid that suitable for Agrobacterium-mediated transformation pUNDV was constructed, which carried fussion protein gene of Newcastle disease virus(NDV-F) under the control of ubiquitin(Ubi) promoter, the selectable marker hygromycin phosphotranferase gene(HPT) and the reporter glucuronidase gene(GUS). Trangenic rice plants were obtained via Agrobacterium-mediated transformation. Most of these transgenic plants were confirmed by PCR analysis and GUS activity detection, and the results indicated that T-DNA containing NDV-F gene had been integrated into the genome of transgenic rice plants, which lay the base for the further study on the production of new castle disease virus gene engineering vaccine in transgenic rice.