| 鸟苷补料分批发酵的研究 |
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| 引用本文： | 赵颖娟,武改红,陈宁.鸟苷补料分批发酵的研究[J].生物技术通讯,2006,17(1):52-54. |
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| 作者姓名： | 赵颖娟 武改红 陈宁 |
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| 作者单位： | 天津科技大学生物工程学院,天津,300222 |
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| 基金项目： | 天津市高等学校科技发展基金项目(20031205) |
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| 摘 要： | 目的:以枯草芽孢杆菌TA208为出发菌株,研究了补料分批发酵方式下各种参数对鸟苷产量的影响。方法:采用补料分批发酵工艺,利用纸层析法测定发酵液中鸟苷的产量。结果:确定了葡萄糖、酵母粉和次黄嘌呤的最优补料方式,使鸟苷产量达到32.05g/L,较分批发酵方式提高了36.3%。结论:发酵工艺过程控制对发酵生产鸟苷具有重大影响。
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| 关 键 词： | 鸟苷 补料分批发酵 分批发酵 枯草芽孢杆菌 |
| 文章编号： | 1009-0002（2006）01-0052-03 |
| 收稿时间： | 2005-06-22 |
| 修稿时间： | 2005年6月22日 |
Studies on Fed-Batch Fermentation of Guanosine |
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| ZHAO Ying-juan,WU Gai-hong,CHEN Ning.Studies on Fed-Batch Fermentation of Guanosine[J].Letters in Biotechnology,2006,17(1):52-54. |
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| Authors: | ZHAO Ying-juan WU Gai-hong CHEN Ning |
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| Abstract: | Objective Using Subtillis bacillus TA208 as production strain the affects of different fed-batch fermentation styles on the guanosine productivity were studied. Methods Using fed-batch fermentation styles guanosine concentration in fermented broth was determined with methods of paper motlle elution colorimetry. Results The optimal fed-batch fermentation condition was determinedby flowing the carbon source and nitrogen source. The productivity of guanosine increased to 35.05 g/L which increased by 36.3% of the batch fermentation. Conclusion The affects of different fed-batch fermentation styles on the guanosine productivity were very important. |
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| Keywords: | guanosine fed-batch fermentation batch fermentation Subtillis bacillus |
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