| 用酵母双杂交系统研究SMAD3和SMAD4的相互作用 |
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| 引用本文： | 刘一平,邓继先,蒋忠军,程萱,黄翠芬.用酵母双杂交系统研究SMAD3和SMAD4的相互作用[J].生物技术通讯,1998(3). |
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| 作者姓名： | 刘一平 邓继先 蒋忠军 程萱 黄翠芬 |
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| 作者单位： | 军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，广州军区军事医学研究所，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所 北京 100071，北京 100071，北京 100071，北京 100071 |
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| 摘 要： | 利用酵母双杂交试验,鉴定了细胞内信号传导蛋白SMAD3和SMAD4的相互作用。通过SMAD3和SMAD4各突变体的同源和异源相互作用的双杂交反应,确定SMAD4介导信号传递的功能区在中间连接区,SMAD3的功能区在C末端。
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| 关 键 词： | SMAD3 SMAD4 酵母双杂交 |
Interaction between SMAD3 and SMAD4 with yeast two-hybrid |
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| Liu Yiping,Deng Jixian,Jiang Zhongjun,Cheng Xuan,Huang Cuifen.Interaction between SMAD3 and SMAD4 with yeast two-hybrid[J].Letters in Biotechnology,1998(3). |
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| Authors: | Liu Yiping Deng Jixian Jiang Zhongjun Cheng Xuan Huang Cuifen |
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| Abstract: | The interaction between SMAD3 and SMAD4 , two intracellular signaling proteins, was identified with yeast two-hybrid system. Several mutants of SMAD3 and SMAD4 constructed, were cloned with pGAD424 and pGBT9 plasrnids simultaneously. The yeast Hf7C was transfected with the cloned plasmids by pair. It appeared that the functional domain of SMAD4 was in the linker region, while that of SMAD3 was in the C terminal. |
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| Keywords: | SMAD3 SMAD4 yeast two-hybrid |
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