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| 重组依赖辅酶B12的甘油脱水酶基因表达系统构建 | |
| 引用本文： | 邵敬伟,刘长江,吕淑霞,吴志香.重组依赖辅酶B12的甘油脱水酶基因表达系统构建[J].生物技术通讯,2004,15(1):13-16. |
| 作者姓名： | 邵敬伟 刘长江 吕淑霞 吴志香 |
| 作者单位： | 1. 沈阳农业大学,食品学院,沈阳,110161 2. 沈阳农业大学,生物技术学院,沈阳,110161 3. 辽宁中医学院,附属医院,沈阳,110032 |
| 摘 要： | 采用PCR方法从肺炎克雷伯氏杆菌基因组中分别扩增得到了编码依赖辅酶B12的甘油脱水酶α、β、γ三个亚基的基因gldA、gldB、gld将gldBC基因克隆至pSIM—T载体上，经测序正确后亚克隆至pGEX-4T-1载体中。将gldA进行T-A克隆后测定核苷酸序列正确，亚克隆至连有gldBC基因的pGEX-4T-1载体中。从而成功的构建了gldABC基因的同向串联原核融合表达载体pGEX—gldABC。 |
| 关 键 词： | 肺炎克雷伯氏杆菌 依赖辅酶B12甘油脱水酶 pGEX-4rr-l融合表达载体 核苷酸序列 |
| 文章编号： | 1009-0002(2004)01-0013-04 |
| 修稿时间： | 2003年7月9日 |
Construction of the expression system of recombinant coenzyme B12-dependent glycerol dehydratase gene |
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| SHAO Jing-wei ,LIU Chang-jiang ,LV Shu-xia ,WU Zhi-xiang .Food Science College,Shenyang Agricultural University,Shenyang,.Biology College,Shenyang Agricultrual University,Shenyang,.Liaoning Traditional Medical University,Shenyang,China.Construction of the expression system of recombinant coenzyme B12-dependent glycerol dehydratase gene[J].Letters in Biotechnology,2004,15(1):13-16. | |
| Authors: | SHAO Jing-wei LIU Chang-jiang LV Shu-xia WU Zhi-xiang Food Science College Shenyang Agricultural University Shenyang Biology College Shenyang Agricultrual University Shenyang Liaoning Traditional Medical University Shenyang China |
| Institution: | SHAO Jing-wei 1,LIU Chang-jiang 1,LV Shu-xia 2,WU Zhi-xiang 3 1.Food Science College,Shenyang Agricultural University,Shenyang110161,2.Biology College,Shenyang Agricultrual University,Shenyang110161,3.Liaoning Traditional Medical University,Shenyang110032,China |
| Abstract: | The gldA,gldB,gldC gene coding glycerol dehydratase was amplified by PCR using the genomic DNA of Kleb-siella pneumoniae as the template,and the gldBC gene was cloned into vector pSIM-T.After the DNA sequence was de-termined,The gldBC gene was subcloned into expression vector pGEX-4T-1,the recominend plasmid was named as pGEX-gldBC.The nucleotide sequence of the gldA was sequenced after T-A cloning,gldA was subcloned into expression vector pGEX-gldBC.The expression plamid pGEX-gldABC was constructed successfully. |
| Keywords: | Klebsiella pneumoniae coenzyme B 12 -dependent glycerol dehydratase pGEX-4T-1fusion expression vector |
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