| 出芽短梗霉新菌株RM1603产普鲁兰多糖条件优化及多糖分析 |
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| 引用本文： | 沈琦,张殿鹏,郝雅荞,罗翔莲,杨佳瑶,魏步云,李欧,赵洪新.出芽短梗霉新菌株RM1603产普鲁兰多糖条件优化及多糖分析[J].生物技术通讯,2019(3):385-390. |
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| 作者姓名： | 沈琦 张殿鹏 郝雅荞 罗翔莲 杨佳瑶 魏步云 李欧 赵洪新 |
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| 作者单位： | 浙江理工大学生命科学学院浙江省植物与次生代谢重点实验室;北京市农林科学院植物保护环境保护研究所;沈阳师范大学生命科学学院 |
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| 基金项目： | 国家农业部“减肥减药”作物免疫调控与物理防控技术及产品研发项目(2017YFD0200908);浙江省科技厅公益技术应用研究计划(2017C32050) |
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| 摘 要： | 目的:出芽短梗霉RM1603是一株高产胞外多糖新菌株,通过优化其产糖条件,鉴定其多糖结构,为进一步开发利用RM1603产胞外多糖奠定理论基础。方法:以RM1603为出发菌株,在单因素分析确定最佳氮源与无机盐的基础上,利用正交试验探究RM1603最佳发酵产糖条件;薄层层析及红外光谱分析确定胞外多糖产物结构。结果:出芽短梗霉菌RM1603的初始多糖产量为28.91g/L,发酵条件优化后产糖量达到65.213g/L,提高了约2.3倍;结构分析表明RM1603的多糖产物为普鲁兰多糖。结论:出芽短梗霉RM1603是一株具有较大产糖优势,极具开发潜力的高产普鲁兰糖新菌株;奠定了开发利用RM1603生产普鲁兰多糖的理论基础。
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| 关 键 词： | 出芽短梗霉菌 普鲁兰多糖 正交试验 薄层层析 红外光谱 |
Optimum Conditions and Identification of Pullulan Produced by a New Finding Strain Aureobasidium pullulans RM1603 |
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| SHEN Qi,ZHANG Dian-Peng,HAO Ya-Qiao,LUO Xiang-Lian,YANG Jia-Yao,WEI Bu-Yun,LI Ou,ZHAO Hong-Xin.Optimum Conditions and Identification of Pullulan Produced by a New Finding Strain Aureobasidium pullulans RM1603[J].Letters in Biotechnology,2019(3):385-390. |
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| Authors: | SHEN Qi ZHANG Dian-Peng HAO Ya-Qiao LUO Xiang-Lian YANG Jia-Yao WEI Bu-Yun LI Ou ZHAO Hong-Xin |
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| Institution: | (Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018;Institute of Plant Protection and Environmental Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097;College of Life Science, Shenyang Normal University, Shenyang 110034, China) |
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| Abstract: | SHEN Qi;ZHANG Dian-Peng;HAO Ya-Qiao;LUO Xiang-Lian;YANG Jia-Yao;WEI Bu-Yun;LI Ou;ZHAO Hong-Xin(Zhejiang Province Key Laboratory of Plant Secondary Metabolism and Regulation, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018;Institute of Plant Protection and Environmental Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097;College of Life Science, Shenyang Normal University, Shenyang 110034, China) |
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| Keywords: | Aureobasidium pullulans pullulan orthogonal test thin layer chromatography Fourier transform infrared spectroscopy |
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