| 抗TPO单克姓抗体杂交瘤细胞系的建立 |
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| 引用本文： | 于芳,朱恒奇,陈琳,徐秀英,黄培堂.抗TPO单克姓抗体杂交瘤细胞系的建立[J].生物技术通讯,2001,12(2):112-113. |
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| 作者姓名： | 于芳 朱恒奇 陈琳 徐秀英 黄培堂 |
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| 作者单位： | 军事医学科学院生物工程研究所 |
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| 摘 要： | 用合成肽TPO作抗原，经腹腔免疫Bal b/c小鼠，鼠抗血清效价为1：1000，ELISA间接法测定的P／N值为3。取小鼠脾细胞与骨髓瘤细胞（SP2／10）在PEG作用下进行融合，细胞融合率达91％。通过ELISA筛选并经过3次亚克隆，获得了1株能分泌抗TPO抗体的单克姓杂交瘤细胞株，P／N值均高于8。
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| 关 键 词： | 血小板生成素 单克隆抗体 杂交瘤 酶联免疫吸附试验 TPO |
| 修稿时间： | 2000年12月7日 |
The preliminary determination of anti-TPO monoclonal antibody hybridoma cell line |
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| YU Fang,ZHU Heng-Qi,CHEN Lin,XU Xiu-ying,HUANG Pei-tang.The preliminary determination of anti-TPO monoclonal antibody hybridoma cell line[J].Letters in Biotechnology,2001,12(2):112-113. |
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| Authors: | YU Fang ZHU Heng-Qi CHEN Lin XU Xiu-ying HUANG Pei-tang |
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| Abstract: | Bal b/c mice were immunized with TPO antigen from synthesized peptide, their spleen cells were fused with myeloma cells by polyethyleneglyco (PEG)making for hybridomas cell line. The rate of fusion was 91%. By making ELISA screening and subcloning, one hybridoma cell line was established. |
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| Keywords: | thrombopoietin monoclonal antibody hybridoma ELISA |
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