| 蛋白质组学研究中的双向电泳技术 |
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| 引用本文： | 廖翔,应天翼,黄留玉,黄培堂.蛋白质组学研究中的双向电泳技术[J].生物技术通讯,2003,14(6):522-524. |
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| 作者姓名： | 廖翔 应天翼 黄留玉 黄培堂 |
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| 作者单位： | 军事医学科学院,生物工程研究所,北京,100071 |
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| 摘 要： | 蛋白质组学研究已经成为后基因组时代的研究热点，其两大支柱是双向凝胶电泳技术和生物质谱技术。尽管双向电泳技术近几年已经取得了突破性进展，是当前蛋白质分离的最常用技术，但其本身还有一些难以克服的问题。随着质谱技术的快速发展，双向电泳逐渐成为蛋白质组学研究的瓶颈。本综述双向电泳主要技术步骤的现状、存在问题及其改进方向。
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| 关 键 词： | 蛋白质组学 双向电泳 生物质谱技术 生物信息学 |
| 文章编号： | 1009-0002(2003)06-0522-03 |
| 修稿时间： | 2003年5月9日 |
Two-dimensional electrophoresis technology in proteomics |
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| LIAO Xiang,YING Tian-yi,HUANG Liu-yu,HUANG Pei-tang.Two-dimensional electrophoresis technology in proteomics[J].Letters in Biotechnology,2003,14(6):522-524. |
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| Authors: | LIAO Xiang YING Tian-yi HUANG Liu-yu HUANG Pei-tang |
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| Abstract: | Proteomics research has gradually become spotlight in era of post-genomics, with two-dimensional (2-D) electrophoresis and mass spectrometry being its basic. Although as most frequently used technology of proteins separation two-dimensional electrophoresis has broken threshold in recent years, there are still several problems hard to be overcome. Compared to quick progress of mass spectrometry technology, 2-D electrophoresis had become bottleneck of proteomics research. Recent developments of 2-D electrophoresis technology are reviewed in this letter. |
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| Keywords: | proteomics two-dimensional electrophoresis |
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