新型脂肽类辐射防护剂H6101对小鼠骨髓细胞基因表达谱的影响

目的：脂肽类化合物具有抗辐射活性，通过研究脂肽类辐射防护剂H6101给药后小鼠骨髓细胞基因表达谱的变化，揭示其可能的辐射防护机制。方法：6只ICR雄性小鼠随机分为PBS对照组和H6101给药组，每组3只，给药后1h分离骨髓细胞提取总RNA，经反转录和荧光标记后与小鼠基因表达谱芯片杂交，杂交信号经扫描仪捕获后用Genenomestudio软件进行统计分析。结果：在测定的26766个基因中，给药组与对照组之间的2倍差异表达基因为1738个，其中1041个基因表达上调，697个基因表达下调；TLR信号通路相关基因，炎性细胞因子、造血因子和细胞凋亡相关基因等的转录水平发生明显变化。结论：用基因表达谱芯片筛选出许多不同种类的与H6101辐射防护作用有关的重要基因，这为揭示脂肽分子辐射防护机制提供了研究方向。

Effects of Lipopeptide Radioprotective Agent H6101 on Gene Expression Profile in Mouse Bone Marrow Cells

Objective： The lipopeptide have the activity of radioprotection. To reveal the radioprotective mechanism of lipopeptide H6101 in mouse, a cDNA microarrary assay was carried out to detect the effects on gene expression in mouse bone marrow eells（BMC） after H6101 administration. Methods： 6 mice were randomly divided into phosphate buffer saline control group and H6101 administration group, 1 hour later, the bone marrow cell were collected respectively, total RNA was extracted and then reverse transcribed. The probes were prepared by labeling, the arrays were hybridized against the cDNA probe mixture and the fluorescent signals were scanned. Microarray analysis was performed by using mouse WG-6 v2.0 Expression BeadChip. The obtained data were analyzed with Genenomestudio software. Results： Among the 26 766 tested genes, 1738 genes were found to be different by more than 2-fold between the groups. Moreover, 1041 genes were up-regulated and 697 genes were down-regulated. These genes including TLR signal pathway associated genes, inflammatory and apoptosis associated proteins. Conclusion： Profile gene chip can be used to screen out many different kinds of genes that may participate in the radioprotection of H6101. The new technology may play an important role in the discovery of the gene level mechanism of lipopeptide in the future.