微生物全基因组测序组装中的g印填补方法

目的：研究和开发高通量测序全基因组组装过程中的填补gap的方法。方法：研究组装软件的算法，使用Perl语言编写自动填补gap的程序，并建立全基因组组装的流程。结果：提出了填补gap的末端延伸法，并使用Perl语言进行了编程；在对立克次体高通量测序的组装过程中，这些方法能大大减少gap的数量。结论：本研究提出的末端延伸法能够高效填补全序列组装过程中出现的gap，具有很强的实用性。

Method of Gap Filling in Microbial Sequencing Whole Genome Assembly

Objective： To research and develop gap filling method of high-throughput sequencing genome assembly. Methods： Learn the algorithms of the available assembly software, then write an Perl program to fill the gaps automatically and create a process of whole-genome assembly. Results： In this study we propose end-extending method, which had been written in Perl program, to fill the gap. In the Ricketsia assembly process, gaps can be greatly reduced. Conclusion： The method presented in this study can effectively fill the gaps during the whole gehome sequence assembly, with high practicability.