水动力转染基因在小鼠体内的长期高效表达

水动力转染技术是近年新出现的一种体内基因转染方法，可以实现目的基因在小鼠体内的高效表达，有可能作为受精卵显微注射的一种简单替代技术。但该技术的缺点是转染基因多瞬时表达，外源基因在体内高效表达的时间通常不超过1周，而且局限于肝、肾等器官，从而限制了该技术广泛应用。为了延长水动力转染基因在小鼠体内长期高效表达的时间，从而能对目的基因的体内功能进行长期研究，国外研究进行了广泛的探索，本综述了相关研究的最新进展。

Long-term and High Efficient Expression of Hydrodynamics Transfected Genes in vivo

Hydrodynamics based transfection is a recently developed technique of gene transfer in vivo. It can be used to efficiently express a foreign gene in mouse and it is possible to replace zygote microinjection to study function of gene in vivo for a long time. But there are some disadvantages for this method limiting its widely use, such as expression of the transfected gene lasting for a short period, usually no longer than one week, and their expression only in liver. In this paper recent development in methods which can make the hydrodynamics transfected gene long-term and efficient expression was reviewed.