不同浓度卡那霉素、潮霉素对楸树试管苗生长的影响

目的:将不同质量浓度的卡那霉素、潮霉素加入楸树培养基中,研究卡那霉素、潮霉素对楸树组培苗生长的影响,以确定抗生素对楸树茎段分化与生根的敏感质量浓度。方法:待楸树继代、生根培养基灭菌后温度降至30~50℃,将不同质量浓度的卡那霉素、潮霉素经抽滤式灭菌加入培养基中,在培养基中接入楸树组培无菌茎段培养,观测茎段继代(增殖芽数、芽长、叶数等)、生根(发根数、根长、芽长等)生长指标并统计分析。结果:楸树组培继代培养基添加卡那霉素质量浓度为100 mg/L时组培瓶苗生长缓慢,浓度为150 mg/L时叶片大部分发白并干枯,茎段基部无愈伤组织形成,瓶苗基本停止生长,楸树继代瓶苗对卡那霉素耐受性范围为100~150 mg/L;添加潮霉素质量浓度为5 mg/L时瓶苗生长较为缓慢,浓度为10 mg/L时叶片开始干枯,茎段基部愈伤组织较小,瓶苗基本停止生长,楸树继代瓶苗对潮霉素耐受性范围为10 mg/L左右。楸树组培生根培养基添加卡那霉素质量浓度为100 mg/L时大部分茎段干枯,少部分为绿但未分化芽与根,浓度为150 mg/L时大部分茎段干枯,极少上部为绿,基部干枯,但未分化芽与根,楸树组培瓶苗生根培养苗对卡那霉素耐受性范围为100~150 mg/L;添加潮霉素质量浓度为5 mg/L时少部分茎段干枯,浓度为10 mg/L时大部分茎段干枯,少部分为绿,茎段未出现芽的分化与根的萌发现象,楸树组培瓶苗生根培养苗对潮霉素耐受性范围为5~10 mg/L。结论:卡那霉素、潮霉素对楸树组培苗生长有明显的抑制作用且与抗生素浓度呈负相关,但低质量浓度(1 mg/L)的潮霉素对楸树继代分化芽数有促进作用;同一抗生素对楸树不同无性系间组培苗生长的影响无显著差异。

Effect of the Kanamycin and Hygromycin on Catalpa bungei in Vi-tro Culture

Objective： To study the effect of kanamycin and hygromycin on the growth of tissue culture seedling of Catalpa bungei, the different concentrations of kanamycin and hygromycin were added into the subculture and rooting medium. We determined the concentration of antibiotic sensitivity of C.bungei subculture and rooting of stem segment. Methods： The different concentrations of kanamycin and hygromycin were added into the medium of the C.bungei subculture with filtration sterilization. The sterile stems were cultured in the medium, and the growth index such as bud length, bud number, leaf number etc. in subculture, and root number, root length, shoot length and so on in root were observed and statistical analyzed. Results： Experiments showed that C.bungei plant？let grew slowly when kanamycin concentration was 100 mg/L in subculture medium. When the kanamycin concen？tration was 150 mg/L, most of the leaves turned white and dried up, and the stem base was without callus, so tol？erance range of appropriate concentration to subculture was 100~150 mg/L. 5 mg/L hygromycin was added into subculture medium, C.bungei plantlet grew slowly. When the concentration reached 10 mg/L, the leaves dried up and stem base callus was smaller and plantlets stoped grow, thus tolerance range of hygromycin appropriate concen？tration to subculture was about 10 mg/L. Most C.bungei stem dried and a few green but not differentiation bud＆amp;nbsp;and root when 100 mg/L kanamycin was added into root medium. When kanamycin concentration was 150 mg/L, C. bungei stems were basic dead and no differentiation bud and root, and rooting seedlings on kanamycin tolerance range of 100~150 mg/L tissue culture plantlets of C.bungei. When hygromycin concentration was 5 mg/L, a few stem dried, at the concentration of 10 mg/L, most stem dried, some turned green, stems were without bud differen？tiation and root germination, seedlings of hygromycin tolerance range was 5~10 mg/L in rooting culture of C. bungei. Conclusion： Experiments showed tha