津田芜菁和赤丸芜菁查尔酮异构酶基因的克隆及表达特性

目的：克隆津田芜菁和赤丸芜菁查尔酮异构酶（CHI）基因并研究其表达特性。方法：利用UV-A处理2种芜菁未见光块根24h，提取总RNA后通过RT-PCR方法克隆津田芜菁和赤丸芜菁的BrCH11和BrCH12基因，通过Northern杂交检测BrCH11和BrCH12基因的UV-A诱导表达特性。结果：BrCH11和BrCH12的开放读码框为756bp，编码251个氨基酸残基；氨基酸序列分析显示，BrCH11和BrCH12与萝卜CHI的同源性达91％，第11-222的肽段具有CHI结构域；BrCH11和BrCH12，2的核苷酸序列和推导的氨基酸序列分别在3个位点存在差异；BrCH11和BrCH12基因具有高度同源性；BrCH11和BrCH12基因的表达量与UV-A处理时间相关。结论：克隆了津田芜菁和赤丸芜菁的BrCH11和BrCH12基因，这2个基因的表达受UV-A诱导。

Cloning and Expression Trait of Chaleone Isomerase Genes in 'Tsuda' and 'Yurugi Akamaru' Turnip

Objective： To clone the chalcone isomerase（CHl） genes of ‘Tsuda＇ and ‘Yurugi Akamaru＇ turnip, and to study the expression trait of the two genes. Methods： The roots of ‘Tsuda＇ and ‘Yurugi Akamaru＇ turnip which grew under dark condition were irradiated with UV-A light for 24h. Total RNA was isolated and then BrCH11 and BrCH12 genes were cloned by RT-PCR method. The expression of BrCH11 and BrCH12 genes was detected by Northern blotting. Results： The open reading frame of BrCH11 and BrCH12 genes contained 756 bp encoding proteins of 251 amino acids. Amino acid sequence analysis showed that BrCH11 and BrCH12 were 91% identity to CHI of Raphanus sativus, and the CHI domain was in the sequence from 11 to 222. The nucleotide sequence and deduced amino acid sequence of BrCH11 and BrCH12 genes had 3 differences. BrCH11 and BrCH12 genes had high identity. The Northern blotting results showed that the expression amount of BrCH11 and BrCH12 was correlated with light-exposure time. Conclusion： BrCH11 and BrCH12 genes of ‘Tsuda＇ and ‘Yurugi Akamaru＇ turnip were cloned successfully, and the expression of the two genes could be induced by UV-A.