| 蛋白质复性研究进展 |
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| 引用本文： | 吴军.蛋白质复性研究进展[J].生物技术通讯,1998(2). |
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| 作者姓名： | 吴军 |
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| 作者单位： | 军事医学科学院生物工程研究所 北京 |
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| 摘 要： | 许多蛋白在大肠杆菌中高效表达时,其产物常以无活性的包含体形式存在,包含体蛋白的复性往往是制备这些蛋白的关键步骤之一,蛋白复性包括肽链折叠和分子内二硫键的氧化这两个互相影响的过程,本文综述了蛋白折叠过程的研究进展,及促进蛋白折叠和二硫键氧化的方法。
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| 关 键 词： | 重组蛋白 复性 蛋白折叠 二硫键氧化 |
Renaturation of recombinant proteins |
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| Wu Jun,Ma Qingjun.Renaturation of recombinant proteins[J].Letters in Biotechnology,1998(2). |
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| Authors: | Wu Jun Ma Qingjun |
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| Abstract: | Expression of recombihant proteins in Escherichia coli often results in the formation of insoluble inclusion bodies. So. renaturation of recombinant proteins was one of the important steps for the activity protein production. The process of prtems renaturation is including peptides refolding and disulphide bond oxidizing which were influenced each other. We reviewed the progress of protein refolding and the method for improving protein refolding and disulphide bonds oxidizing. |
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| Keywords: | recombtnant proteins renaturation protein refolding disulphlde bonds oxidizing |
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