2型猪链球菌假想转运蛋白89k/pTP与毒力的相关分析

目的：构建2型猪链球菌假想转运蛋白89k/pTP的基因插入失活突变体并进行毒力相关分析。方法：首先将同源臂片段克隆到pSET4s质粒上,构建插入失活载体pSET4s-pTP,并通过对单交换的筛选获得89k/pTP的基因插入失活突变体,再用小鼠腹腔感染模型对突变株和野生株的毒力进行比较。结果：获得了89k/pTP的基因插入失活突变体,并发现其毒力与野生型相比明显下降。结论：2型猪链球菌假想转运蛋白89k/pTP与其毒力有关,其作用和机制值得进一步分析。

Analysis of the Correlation Between the Putative Transporter 89k/pTP and Virulence for Streptococcus suis Serotype 2

Objective： To construct insertional inactivation mutant of the putative transporter 89k/pTP of Streptococcus suis serotype 2 and to give a correlation analysis for its virulence. Methods： The homologous ann was cloned into suicide vector pSET4s to build inactivation plasmid pSET4s-pTP, and then the insertional inactivation mutant 89k/pTP which was aquired by verifing the single-exchange clones was obtained. The comparison of the mutant and wild type was analysed by the mice infection model. Results： The insertional inactivation mutant of 89k/pTP was constructed successfully and its virulence attenuated significantly than wild type. Conclusion： The putative transporter 89k/pTP contributes to the virulence of S.suis serotype 2, and the role and mechanism of this protein is valuable for futher analysis.