| PCR快速鉴定鼠疫耶尔森氏菌 |
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| 引用本文： | 于学东,裴德翠,周东生,宋亚军,郭兆彪,王津,杨瑞馥.PCR快速鉴定鼠疫耶尔森氏菌[J].生物技术通讯,2003,14(6):569-572. |
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| 作者姓名： | 于学东 裴德翠 周东生 宋亚军 郭兆彪 王津 杨瑞馥 |
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| 作者单位： | 军事医学科学院,微生物流行病研究所,北京,100071 |
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| 摘 要： | 建立一种简便、快速、特异的PCR检测方法，用于鼠疫耶尔森氏菌的快速鉴定。针对鼠疫耶尔森氏菌特异的一段染色体序列3a设计引物，扩增-276bp片段的鼠疫标识序列。应用该PCR反应体系，对我国17个生态型及1个待定的生态型共计275株鼠疫耶尔森氏菌及48株相关菌株的PCR扩增结果表明，实验菌株均扩增出预期的276bp片段产物带，48株相关菌株均阴性，其检测灵敏度为100pg DNA。说明该方法用于鼠疫耶尔森氏菌的检测鉴定简便、快捷，具有很高的特异性和敏感性。
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| 关 键 词： | 鼠疫耶尔森氏菌 PCR 标识序列 引物设计 |
| 文章编号： | 1009-0002(2003)06-0569-04 |
| 修稿时间： | 2003年8月19日 |
Rapid identifition of Yersinia pestis by PCR |
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| YU Xue-dong,PEI De-cui,ZHOU Dong-sheng,SONG Ya-jun,GUO Zhao-biao,WANG Jin,YANG Rui-fu.Rapid identifition of Yersinia pestis by PCR[J].Letters in Biotechnology,2003,14(6):569-572. |
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| Authors: | YU Xue-dong PEI De-cui ZHOU Dong-sheng SONG Ya-jun GUO Zhao-biao WANG Jin YANG Rui-fu |
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| Abstract: | To develop a simple and specific PCR method for rapid identification of Yersinia pestis strains. The PCR assay was based upon primers derived from Y. pestis highly specific region on chromosome to amplify a 276bp product to identify Y. pestis. The selected primers amplified a 276-bp product from all 275 Y. pestis strains tested. No PCR product was generated for closely related bacteria, including Y. pseudotuberculosis, Y. enterocolitica and others in Enterobacteriaceae. This PCR assay was rapid and specific for rapid identification of Y. pestis. it will be useful to identify Y. pestis in case of emergency and for the surveillance of epidemics. |
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| Keywords: | Yersinia pestis' identification signature sequence PCR |
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