| 用MLVA技术和多重PCR对犬种布氏菌基因分型 |
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| 引用本文： | 姜海,崔步云,李兰玉,赵鸿雁,朴东日.用MLVA技术和多重PCR对犬种布氏菌基因分型[J].生物技术通讯,2009,20(3):336-338. |
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| 作者姓名： | 姜海 崔步云 李兰玉 赵鸿雁 朴东日 |
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| 作者单位： | 中国疾病预防控制中心,传染病预防控制所,北京,102206 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 目的：对犬种布氏菌的遗传关系进行不同分子分型方法的对比研究,为犬布病分子流行病溯源提供有效方法。方法：采用多重PCR和多位点可变数量串联重复序列分析（MLVA）方法对24株犬种布氏菌的遗传关系进行比较研究。结果：多重PCR只鉴定出1株犬种布氏菌,其余23株均鉴定为猪种鲁氏菌,但不能鉴定型别;MLVA方法对已鉴定为猪种的布氏菌仍可再细分为型,87%（20/23）为猪3型,13%（3/20）为猪1型。结论：MLVA可以对布氏菌种（生物型）进行基因分型鉴定,可以作为传统表型鉴定方法的补充。
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| 关 键 词： | 犬种布氏菌 多重PCR 多位点可变数量串联重复序列分析 分型 |
Typing Brucella canis Strains Gene with Multiple-Locus Variable-Number Tandem Repeat Analysis and Multiplex PCR Assay |
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| JIANG Hai,CUI Bu-Yun,LI Lan-Yu,ZHA Hong-Yan,PIAO Dong-Ri.Typing Brucella canis Strains Gene with Multiple-Locus Variable-Number Tandem Repeat Analysis and Multiplex PCR Assay[J].Letters in Biotechnology,2009,20(3):336-338. |
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| Authors: | JIANG Hai CUI Bu-Yun LI Lan-Yu ZHA Hong-Yan PIAO Dong-Ri |
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| Institution: | (State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China) |
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| Abstract: | Objective： To study the phylogenesis of Brucella canis with different subtyping and compare the effectiveness of the methods. Methods： 24 B.canis strains were analyzed by multiplex-PCR and multiple-locus variable-number tandem-repeat analysis（MLVA）. Results： 23/24 B.canis strains were defined to B.suis by multiplex-PCR, whereas these B.suis strains were grouped into B.suis bv.3（20/23） and B.suis bv.1（3/23） by MLVA. Conclution： The MLYA assay can be applied to identification of Brucella strains （biotype） and can be proposed to as a complement of classical biotyping methods. |
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| Keywords: | Brucella canis multiplex-PCR multiple-locus variable-number tandem-repeat analysis subtyping |
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