| 小鼠GM-CSF的基因克隆和锚定修饰 |
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| 引用本文： | 于继云,陈兴,蔡欣,田蓉,程绍辉,许红莲,王嘉玺.小鼠GM-CSF的基因克隆和锚定修饰[J].生物技术通讯,2003,14(3):178-179. |
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| 作者姓名： | 于继云 陈兴 蔡欣 田蓉 程绍辉 许红莲 王嘉玺 |
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| 作者单位： | 1. 军事医学科学院,基础医学研究所,北京,100850
2. 第四军医大学,西京医院皮肤科,西安,710032 |
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| 基金项目： | 国家863项目(2001AA217131) |
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| 摘 要： | 将糖基化磷脂酰肌醇(GPI）锚定修饰的细胞因子直接转移到肿瘤细胞膜上，是研究治疗性肿瘤疫苗的一种有潜力的新策略。克隆了小鼠GM-CSF基因，井通过将从衰变加速因子（DAF）来源的GPI修饰性信号序列与mGM—CSF、嵌合，获得了GPI修饰的mGM-CSF分子，构建并鉴定了GPI锚定修饰的真核表达裁体pCI／GPI-mGM-CSF，为进一步研究表面工程治疗性肿瘤疫苗奠定了基础。
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| 关 键 词： | 小鼠 GM－CSF基因 克隆 锚定修饰 肿瘤疫苗 糖基化磷脂酰肌醇 蛋白质修饰 细胞膜 |
| 文章编号： | 1009-0002(2003)03-0178-02 |
| 修稿时间： | 2003年1月10日 |
Cloning mouse GM-CSF and construction of GPI-anchored mGM-CSF |
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| YU Ji-yun ,CHEN Xing ,CAI Xin ,TIAN Rong ,CHENG Shao-hui ,XU Hong-lian ,WANG Jia-xi.Cloning mouse GM-CSF and construction of GPI-anchored mGM-CSF[J].Letters in Biotechnology,2003,14(3):178-179. |
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| Authors: | YU Ji-yun CHEN Xing CAI Xin TIAN Rong CHENG Shao-hui XU Hong-lian WANG Jia-xi |
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| Institution: | YU Ji-yun 1,CHEN Xing 1,CAI Xin 1,TIAN Rong 2,CHENG Shao-hui 1,XU Hong-lian 1,WANG Jia-xi 1 |
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| Abstract: | Direct modification of tumor cell membranes by transfer of a glycosyl-phosphatidylinositol(GPI)-anchored cytokine has been proposed as a novel potentially useful strategy to development of therapeutic tumor vaccines.In this study,mouse GM-CSF was amplified by RT-PCR using the total RNA extracted from BALB/c mouse spleen cells activated with IL-2and anti-CD3antibody in vitro.Glycosylphosphatidylinositol(GPI)-anchored variants of mGM-CSF were produced via chimerization with alternative GPI-modification signal sequences from decay-accelerat-ing factor(DAF).GPI-anchored mGM-CSF eukaryotic expression vector pCI /GPI-mGM-CSF was contracted and iso-latedand,it will may be used for research of new type therapeutic tumor vaccines. |
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| Keywords: | cancer vaccine GM-CSF glycosyl-phosphatidyl inositol-anchored protein cell-surface engineering |
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