| 炭疽芽孢杆菌保护性抗原在大肠杆菌中的表达及纯化 |
| |
| 引用本文： | 展德文,刘向昕,陶好霞,王令春,张兆山.炭疽芽孢杆菌保护性抗原在大肠杆菌中的表达及纯化[J].生物技术通讯,2005,16(5):503-504. |
| |
| 作者姓名： | 展德文 刘向昕 陶好霞 王令春 张兆山 |
| |
| 作者单位： | 军事医学科学院,生物工程研究所,北京,100071 |
| |
| 摘 要： | 按照炭疽芽孢杆菌保护性抗原（PA）基因成熟肽编码序列设计引物，从炭疽杆菌pOX1质粒中扩增出PA基因片段，将该片段定向插入到原核表达载体pET-28a中，获得了pET-PA原核表达重组质粒，限制性酶切分析和DNA序列测定均证实该克隆插入片段为PA基因的成熟呔编码序列。将该重组质粒转化大肠杆菌BL21（DE3），经IPTG诱导，重组蛋白在大肠杆菌表达系统中获得了高效表达；Western印迹分析表明表达产物具有良好的免疫学活性。
|
| 关 键 词： | 炭疽杆菌 保护性抗原 表达 |
| 文章编号： | 1009-0002(2005)05-0503-02 |
| 收稿时间： | 03 16 2005 12:00AM |
| 修稿时间： | 2005年3月16日 |
Expression and Purification of the Recombinant Protective Antigen of Bacillus anthracis in Escherichia coli |
| |
| ZHAN De-wen,LIU Xiang-xin,TAO Hao-xia,WANG Ling-chun,ZHANG Zhao-shan.Expression and Purification of the Recombinant Protective Antigen of Bacillus anthracis in Escherichia coli[J].Letters in Biotechnology,2005,16(5):503-504. |
| |
| Authors: | ZHAN De-wen LIU Xiang-xin TAO Hao-xia WANG Ling-chun ZHANG Zhao-shan |
| |
| Institution: | Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China |
| |
| Abstract: | The primers specific for the protective antigen(PA) of Bacillus anthracis coding sequence was designed and synthesized. The PA gene was cloned from pOX1 plasmid by using PCR and inserted into vector pET-28a to obtain the recombinant expressing plasmind pET-28a-PA. The restriction enzyme analysis and DNA sequence detection confirmed that the inserted fragment of clone pET-28a-PA is the mature PA coding sequence. The recombinant DNA was transformed into the host cells E.coli BL21(DE3). The BL21(DE3) with pET-PA was induced with IPTG. The fusion protein effective expressed. The result of Western-blot showed that this fusion protein reacted specifically to the monoclonal antibodies to PA. |
| |
| Keywords: | Bacillus anthracis protective antigen expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
