EphA3受体截短突变体variant b在前列腺癌细胞中的分泌表达

目的:验证EphA3受体截短突变体variant b是否是一种分泌蛋白,并探索其内源分泌的特点,在细胞水平看其是否有作为前列腺癌血清标志物的潜质。方法:构建EphA3 variant b真核表达载体,应用标签抗体、特异性抗体和Western印迹检测前列腺癌细胞培养液中EphA3 variant b的表达,用RT-PCR方法分析EphA3 variant b在8种细胞系中的表达谱和对雄激素刺激的应答。结果:验证了EphA3 variant b是一种分泌蛋白,在雄激素受体阳性的前列腺癌细胞系中特异性表达,雄激素以剂量依赖方式诱导EphA3 variant b表达。结论:EphA3 variant b是一种分泌蛋白,其表达与雄激素受体信号通路相关,有作为前列腺癌血清标志物的潜质。

Expression and Secretion Characteristic of EphA3 Variant b in Prostate Cancer Cell Lines

Objective:To validate the truncated mutant of EphA3 receptor(EphA3 variant b)a secretary protein and to explore its endogenous secretion character,as well as to test if it can be used as a serum biomarker for prostate cancer on cellular level.Methods:By constructing eukaryotic expression vector and Western blotting with anti-His tag antibody as well as specific antibody,EphA3 variant b was tested if it can be expressed in cell culture fluid of prostate cancer cell lines.Then the expression spectrum of EphA3...