| G蛋白亲和色谱法纯化动物血清、抗体效果比较 |
| |
| 引用本文： | 邓瑞春,白云秀,张明伟,汪劲松,黄英,毛宁.G蛋白亲和色谱法纯化动物血清、抗体效果比较[J].生物技术通讯,1999,10(3):198-200. |
| |
| 作者姓名： | 邓瑞春 白云秀 张明伟 汪劲松 黄英 毛宁 |
| |
| 作者单位： | 北京瑞得四环生物技术研究所,北京,100850 |
| |
| 摘 要： | 比较 Hitrap G蛋白 Sepharose亲和色谱系统对不同动物血清或腹水的纯化效果 ,为抗体纯化提供依据。结果显示 ,G蛋白对不同动物血清 Ig G吸附能力不同 ,体现在单位体积抗体回收量明显不同 ,这一特点与 A蛋白亲和色谱系统相似。该方法简便快速 ,纯化抗体纯度高 ,免疫活性好 ,色谱柱可反复使用多次
|
| 关 键 词： | G蛋白 A蛋白 亲和色谱法 抗体 |
Comparison of IgG purification from different animal sera by Hitrap protein G affinity chromatography |
| |
| Deng Ruichun,Bai Yunxiu,Zhang Mingwei,Wang Jingsong,Huang Ying,Mao Ning.Comparison of IgG purification from different animal sera by Hitrap protein G affinity chromatography[J].Letters in Biotechnology,1999,10(3):198-200. |
| |
| Authors: | Deng Ruichun Bai Yunxiu Zhang Mingwei Wang Jingsong Huang Ying Mao Ning |
| |
| Institution: | Deng Ruichun,Bai
Yunxiu,Zhang Mingwei,Wang Jingsong,Huang Ying,Mao Ning;(Read & Four-Ring Institute of
Biotechnology,Beijing 100850) |
| |
| Abstract: | Several kinds of IgG from different animal sera, orascites were purified by Hitrap protein G affinity chromatography, and their purities were compared. The results showed that Hitrap protein G affinity chromatography was an easy and efficient way to purify IgG. The Hitrap gel could remain working well in absorbing IgG after a long time. But there were some differences among the ability to absorb IgG from different sera. |
| |
| Keywords: | protein G affinity chromatography antibody |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
