| 烟草生产霍乱毒素B亚单位疫苗的免疫分析 |
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| 引用本文： | 王新国,张艳红,刘传暄,高丽华,肖成祖,张国华,方荣祥.烟草生产霍乱毒素B亚单位疫苗的免疫分析[J].生物技术通讯,2000,11(3):192-195. |
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| 作者姓名： | 王新国 张艳红 刘传暄 高丽华 肖成祖 张国华 方荣祥 |
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| 作者单位： | 1. 军事医学科学院生物工程研究所,北京,100071
2. 中国科学院微生物研究所,北京,100080 |
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| 摘 要： | 用纯化的ＣＴＢ后腿肌肉注射免疫小鼠，每次１２．５μｇ，每隔１４天加强一次，共注射三次，末次免疫后两周收集血清，免疫小鼠诱导产生了特异性抗ＣＴＢ抗体。在ＣＨＯ细胞中，免疫小鼠血清能够中和ＣＴ的细胞病变效应；小鼠肠结扎实验表明，抗ＣＴＢ血清能够抑制ＣＴ结合细胞表面受体ＧＭ１神经节苷脂。这表明转基因烟草产ＣＴＢ在小鼠中能够产生抗细菌毒素的保护免疫力。
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| 关 键 词： | 霍乱毒素B亚单位 小鼠 疫苗 免疫 |
Analysis of immunogencity of tobacco-derived cholera toxin B subunit vaccine |
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| Wang Xinguo,Zhang Yanhong,Liu Chuanxuan,Gao Lihua,Xiao Chengzu,Zhang Guohua,Fang Rongxiang.Analysis of immunogencity of tobacco-derived cholera toxin B subunit vaccine[J].Letters in Biotechnology,2000,11(3):192-195. |
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| Authors: | Wang Xinguo Zhang Yanhong Liu Chuanxuan Gao Lihua Xiao Chengzu Zhang Guohua Fang Rongxiang |
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| Abstract: | Plant-derived CTB was used to immunize mice, and the serum samples were examined for CTB-specific antibodies. It indicated that the immunized mice produced significantly high titer of anti-CTB serum. The cytopathic effect of CT on CHO cell was neutralized by the serum, and the protective efficacy of immunization was assayed by mouse ileal loop ligation. All these results demonstrated that the antiserum showed protection against a bacterial enterotoxin. |
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| Keywords: | cholera toxin B subunit mouse vaccine |
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