| C型凝集素LSECtin识别细菌的研究 |
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| 引用本文： | 路彦涛,杨婷,赵向娜,郭兆彪,唐丽,贺福初.C型凝集素LSECtin识别细菌的研究[J].生物技术通讯,2011,22(3):307-311. |
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| 作者姓名： | 路彦涛 杨婷 赵向娜 郭兆彪 唐丽 贺福初 |
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| 作者单位： | 1. 蛋白质组学国家重点实验室,北京蛋白质组研究中心,军事医学科学院,放射医学与辐射医学研究所,北京102206
2. 军事医学科学院,微生物与流行病研究所,北京100071 |
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| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划 |
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| 摘 要： | 目的:通过sLSECtin-Fc蛋白与细菌及细胞的黏附,寻找能与LSECtin结合的细菌。方法:通过ELISA检测sLSECtin-Fc与细菌的黏附,同时构建CHO-pDsRed1-N1-LSECtin稳定细胞株进行与细菌的黏附实验,荧光显微镜进行镜鉴拍照。结果:获得能够与sLSECtin-Fc蛋白黏附的细菌,并且通过过表达LSECtin的稳定株与细菌的黏附,发现LSECtin可以结合大肠杆菌和肺炎链球菌,而不能结合金黄色葡萄球菌和肺炎克雷伯杆菌。结论:发现LSECtin可以结合细菌,为进一步研究LSECtin在先天免疫中的作用奠定了基础。
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| 关 键 词： | C型凝集素 LSECtin 细菌 识别 |
LSECtin, as a C-Type Lectin, Can Recognize Bacteria |
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| LU Yan-Tao,YANG Ting,ZHAO Xiang-Na,GUO Zhao-Biao,TANG Li,HE Fu-Chu.LSECtin, as a C-Type Lectin, Can Recognize Bacteria[J].Letters in Biotechnology,2011,22(3):307-311. |
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| Authors: | LU Yan-Tao YANG Ting ZHAO Xiang-Na GUO Zhao-Biao TANG Li HE Fu-Chu |
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| Institution: | LU Yan-Tao1,YANG Ting1,ZHAO Xiang-Na2,GUO Zhao-Biao2,TANG Li1,HE Fu-Chu11.State Key Laboratory of Protemics,Beijing Proteome Research Center,Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 102206,2.Institute of Microbiology and Epidemiology,Beijing 100071,China |
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| Abstract: | Objective:To investigate whether liver and lymph node sinusoidal endothelial cells lectin(LSECtin)recognizes bacteria by the adhesion assay.Methods:Detecting the adhesion of bacteria to recombinant sLSECtin-Fc protein by ELISA assay,and to the membrane-bound LSECtin stably expressed on the surface of CHO cells by fluorescence microscope assay.Results:Both recombinant sLSECtin-Fc protein and the membrane-bound LSECtin were able to recognize E.coli and Streptococcus pneumoniae,but can not recognize S.aureus a... |
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| Keywords: | LSECtin |
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