| β淀粉样蛋白人源性抗体的制备 |
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| 引用本文： | 孙超,常红艳,王双,林建波,俞炜源,陈燕侠.β淀粉样蛋白人源性抗体的制备[J].生物技术通讯,2010,21(5):681-685. |
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| 作者姓名： | 孙超 常红艳 王双 林建波 俞炜源 陈燕侠 |
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| 作者单位： | 1. 军事医学科学院,生物工程研究所,北京,100071
2. 空军总医院,高干科,北京,100142 |
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| 摘 要： | 目的:应用噬菌体展示及抗体库技术,制备并鉴定β淀粉样蛋白(Aβ)人源性抗体。方法:应用固相筛选方法,以人工合成的Aβ1-15肽为靶标分子,在大容量全合成人源性噬菌体抗体库中筛选抗Aβ人源性抗体,并进行特异性鉴定。结果:经过3轮筛选,单克隆鉴定获得噬菌体抗体F11,竞争性ELISA表明抗体对Aβ1-42的结合位点位于1～15氨基酸残基内,ELISA结果证实抗体特异性良好。结论:以Aβ1-15肽为靶标分子获得了特异性良好的人源性抗体。
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| 关 键 词： | 阿尔茨海默病 β淀粉样蛋白 噬菌体抗体筛选 特异性 |
Preparation and Identification of Human Anti-Amyloid β Peptide Monocional Antibodies |
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| SUN Chao,CHANG Hong-Yan,WANG Shuang,LIN Jian-Bo,YU Wei-Yuan,CHEN Yan-Xia.Preparation and Identification of Human Anti-Amyloid β Peptide Monocional Antibodies[J].Letters in Biotechnology,2010,21(5):681-685. |
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| Authors: | SUN Chao CHANG Hong-Yan WANG Shuang LIN Jian-Bo YU Wei-Yuan CHEN Yan-Xia |
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| Institution: | 1.Beijing Institute of Biotechnology,Beijing 100071;2.Cadre Ward General Hospital of Air Force,Beijing 100142;China |
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| Abstract: | Objective： To preparation anti-amyloid β peptide（Aβ） antibodies from human phage display antibody library.Methods： Aβ1-15 peptide was used for screening specific antibodies from a large fully synthetic human antibody library by solid panning.The antigen binding activity and specificity of antibody were determined by ELISA.Results： After 3 rounds of panning,one candidate（F11） was obtained.The epitope was located within Aβ1-15,and the specificity of F11 was shown to be excellent both in phage-antibody level and whole antibody level by ELISA.Conclusion： Human anti-Aβ1-15 specificity antibodies can be isolated from phage antibody library. |
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| Keywords: | Alzheimer＇ disease amyloid β peptide phage display screening specificity |
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