| TT病毒重组蛋白单克隆抗体的制备 |
| |
| 引用本文： | 刘树玲,周育森,陈万荣,王海涛.TT病毒重组蛋白单克隆抗体的制备[J].生物技术通讯,2001,12(1):32-33. |
| |
| 作者姓名： | 刘树玲 周育森 陈万荣 王海涛 |
| |
| 作者单位： | 军事医学科学院微生物流行病研究所 |
| |
| 基金项目： | 国家自然科学基金资助项目(39900133) |
| |
| 摘 要： | 采用杂交瘤技术，获得了4株稳定分泌抗TTV重组蛋白的单克隆抗体杂交瘤细胞株，1株属IgG2bλ链、1株属IgG1κ链、2株属IgG2aκ链。4株杂交瘤细胞培养上清液效价为1：80-1：1280，腹水效价为1：32万-1：160万。
|
| 关 键 词： | TT病毒 单克隆抗体 酶联免疫吸附实验 重组蛋白 |
| 修稿时间： | 2000年8月4日 |
Preparation and
identification of monoclonal antibodies against recombinant protein of TT virus |
| |
| LIU SHULING,ZHOU Yusen,CHEN Wanrong,WANG HaiTao.Preparation and
identification of monoclonal antibodies against recombinant protein of TT virus[J].Letters in Biotechnology,2001,12(1):32-33. |
| |
| Authors: | LIU SHULING ZHOU Yusen CHEN Wanrong WANG HaiTao |
| |
| Abstract: | Monoclonal antibodies (McAb) against recombinant pr otein of TTV were produced by fusing SP2/0 myeloma cells with spleen cells from Bal b/c mice immunized with TTV recombinant proteins. Four hybridoma cell lines secreting specific antibodies against TTV recombinant proteins were obtained by screening hybridoma culture supernatants using ELISA with TTV recombinant protei ns on solid phase. One of McAbs was IgG2bλ, One of McAbs was IgG1κ, Others wer e IgG2aκ, The titers of hybridoma culture supernatants ranged from 1∶80～1∶1 280 and from 1∶320 000～1∶1 600 000 in ascites. |
| |
| Keywords: | TT virus monoclonal antibody recombinant protein ELISA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
