| 抗细小病毒B19-VP2单克隆抗体的建立 |
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| 引用本文： | 王慧娟,谷淑燕,张素香,周为民,皮国华.抗细小病毒B19-VP2单克隆抗体的建立[J].生物技术通讯,2002,13(6):451-452. |
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| 作者姓名： | 王慧娟 谷淑燕 张素香 周为民 皮国华 |
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| 作者单位： | 中国疾病预防控制中心病毒病预防与控制所,北京,100052 |
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| 摘 要： | 制备抗细小病毒B19－VP2单克隆抗体，用于检测人血清中的B19抗原，辅助诊断相关疾病；也可用于制备人类细小病毒基因工程疫苗。用纯化的基因工程表达的B19－VP2蛋白免疫BALB/c小鼠，取免疫小鼠的脾细胞和小鼠骨髓瘤Sp2/0细胞融合，有限稀释法克隆细胞。ELISA及IF证明抗体特异性。克隆筛选出4株细胞，并初步建立了检测B19－VP2抗原的双抗体夹心酶联免疫吸附试验，为双抗体夹心法检测B19抗原为临床相关疾病诊断提供了检测手段。
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| 关 键 词： | 细小病毒B19－VP2 单克隆抗体 基因工程B19－VP2蛋白 酶联免疫吸附试验 |
| 文章编号： | 1009-0002(2002)06-0451-02 |
| 修稿时间： | 2002年6月2日 |
Establishment of hybridoma secreting monoclonal antibodies to B19-VP2 |
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| WANG Hui-juan,GU Shu-yan,ZHANG Su-xiang,ZHOU Wei-min,PI Guo-hua.Establishment of hybridoma secreting monoclonal antibodies to B19-VP2[J].Letters in Biotechnology,2002,13(6):451-452. |
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| Authors: | WANG Hui-juan GU Shu-yan ZHANG Su-xiang ZHOU Wei-min PI Guo-hua |
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| Abstract: | To establish the monoclonal antibodies of B19(VP2)for the detection of serum B19antigen,as a assis-tant diagnostic method,also can be used for the research of human parvovirus vaccine.BALB/c mice were immu-nized by using purified genetic B19-VP2protein,the hybridoma cell lines were cloned by fusing mice spleen cell with mice myeloma cell and then using limiting dilutions.4hybridoma cell lines were obtained,double-antibodies sandwich ELISA for detecting B19-VP2antigen was established. |
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| Keywords: | genetic B19-VP2protein monoclonal antibodies ELISA |
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