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| 胰岛素样生长因子结合蛋白-1启动子的克隆与序列分析 | |
| 引用本文： | 吴志香,邵敬伟,袁凤山.胰岛素样生长因子结合蛋白-1启动子的克隆与序列分析[J].生物技术通讯,2005,16(6):624-626. |
| 作者姓名： | 吴志香 邵敬伟 袁凤山 |
| 作者单位： | 1. 中国医科大学,第一附属医院内分泌,辽宁,沈阳,110001;辽宁中医学院,附院西医诊断教研室,辽宁,沈阳,110032 2. 福州大学,药物生物技术与工程研究所,福建,福州,350002 3. 中国医科大学,第一附属医院内分泌,辽宁,沈阳,110001 |
| 基金项目： | 辽宁省自然科学基金项日（962293） |
| 摘 要： | 用酚氯仿抽提法提取SD大鼠的肝脏基因组DNA，PCR扩增胰岛素样生长因子结合蛋白-1（IGFBP-1）启动子并克隆至pUC118载体。从含IGFBP-1启动子的质粒中酶切分离出IGFBP-1启动子并测序。PCR扩增出420bp的目的DNA片段，与文献报道的IGFBP-1启动子DNA大小一致。经正、反两方向序列分析显示，克隆的基因序列和GenBank数据库中的IGFBP-1启动子基因序列一致。以上结果表明克隆成功了IGFBP-1启动子基因，为构建具双向调节的胰岛素分泌调控基因质粒奠定了基础。 |
| 关 键 词： | 胰岛素样生长因子结合蛋白-1 1型糖尿病 基因治疗 |
| 文章编号： | 1009-0002(2005)06-0624-03 |
| 收稿时间： | 04 9 2005 12:00AM |
| 修稿时间： | 2005年4月9日 |
Cloning and Identification of the Insulin-like Growth Factor Binding Protein-1 Basal Promoter |
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| WU Zhi-xiang,SHAO Jing-wei,YUAN Feng-shan.Cloning and Identification of the Insulin-like Growth Factor Binding Protein-1 Basal Promoter[J].Letters in Biotechnology,2005,16(6):624-626. | |
| Authors: | WU Zhi-xiang SHAO Jing-wei YUAN Feng-shan |
| Institution: | 1. Department of Endocrinology, The First Affiliated Hospital, China Medical University, Shenyang 110001; 2. Staff Room of Medical Diagnosis, The Affiliated Hospital of Liaoning College of Traditional Chinese Medicine, Shenyang 110032; 3. Institute of Pharmaceutical Biotechnology and Bioengineering, Fuzhou University, Fuzhou 350002, China |
| Abstract: | To clone insulin-like growth factor binding protein-1(IGFBP-1)basal promoter,which can been inhibited by insulin in order to lay groundwork for constructing an insulin gene regulated system stimulated by glucose and inhibited by insulin.The inhibitory insulin response element of the IGFBP-1 basal promoter was amplified by PCR,Choosing pUC118 as the clone vector,the IGFBP-1 basal promoter was got by clone technology.The DNA sequences of the IGFBP-1 cloned were the same as reported previously. |
| Keywords: | insulin-like growth factor binding protein-l type 1 diabetes mellitus gene therapy |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |