| 恶性疟原虫抗原表位的亚单位疫苗与DNA疫苗的联合免疫 |
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| 引用本文： | 钟辉,李平,曹诚,李杰之,马清钧.恶性疟原虫抗原表位的亚单位疫苗与DNA疫苗的联合免疫[J].生物技术通讯,1999,10(2). |
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| 作者姓名： | 钟辉 李平 曹诚 李杰之 马清钧 |
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| 作者单位： | 军事医学科学院生物工程研究所,北京,100850 |
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| 摘 要： | 构建了含有恶性疟原虫抗原基因 ( AWTE)的真核表达质粒 p CMV- AWTE,以及能在大肠杆菌中得到分泌性表达的原核表达质粒 p MC0 5 ,表达的蛋白 AWTE保持了疟原虫抗原的抗原性。将 p CMV- AWTE以及 AWTE两者混合各 1 0μg鼻腔免疫小鼠 ,一次后诱导机体产生了较高水平的体液免疫及细胞免疫
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| 关 键 词： | 恶性疟原虫 DNA疫苗 鼻腔免疫 亚单位疫苗 |
Combined vaccination of DNA vaccine and subunit vaccine of malaria antigen epitope by intranasal route |
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| Zhong Hui,Li Ping,Cao Cheng,Li Jiezhi,Ma Qingjun.Combined vaccination of DNA vaccine and subunit vaccine of malaria antigen epitope by intranasal route[J].Letters in Biotechnology,1999,10(2). |
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| Authors: | Zhong Hui Li Ping Cao Cheng Li Jiezhi Ma Qingjun |
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| Abstract: | Mucosal delivery of DNA vaccine may represent an attractive alternative for future vaccine strategies. In the present studies, we attempted to optimize the vaccination protocol using DNA vaccine, subunit vaccine and their combination form.These date demonstrate that for inducing high titers of malaria antigen specific antibodies and CTL activity, intranasal immunization of combined DNA vaccine and subunit vaccine one time procedures produces better results than immunization with either vaccine alone. |
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| Keywords: | DNA vaccine subunit vaccine nasal immunization |
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