| 重组生物制品十二烷基硫酸钠含量检测方法的建立 |
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| 引用本文： | 王丽华,陈迅,龙应国.重组生物制品十二烷基硫酸钠含量检测方法的建立[J].生物技术通讯,2008,19(5):711-712. |
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| 作者姓名： | 王丽华 陈迅 龙应国 |
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| 作者单位： | 1. 解放军总医院,药品保障中心,北京,100853
2. 北京四环生物制药有限公司,北京,100176 |
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| 摘 要： | 目的：用亚甲基蓝-分光光度法检测重组生物制品中十二烷基硫酸钠（SDS）的含量。方法：将送检样品按比例稀释后与亚甲基蓝混合,加入氯仿进行萃取,然后用紫外分光光度计测定各样品的吸光度D值。结果：SDS浓度为0～0.01mg/mL时,SDS与D651nm值呈线性关系。结论：在一定的SDS浓度范围内可用亚甲基蓝-分光光度法检测重组生物制品中SDS的含量。
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| 关 键 词： | 亚甲基蓝-分光光度法 十二烷基硫酸钠 重组生物制品 |
Establishment of Determination Method of the SDS Contents in Recombinant Biological Products |
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| WANG Li-Hua,CHEN Xun,LONG Ying-Guo.Establishment of Determination Method of the SDS Contents in Recombinant Biological Products[J].Letters in Biotechnology,2008,19(5):711-712. |
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| Authors: | WANG Li-Hua CHEN Xun LONG Ying-Guo |
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| Institution: | WANG Li-Hua, CHEN Xun, LONG Ying-Guo (1.General Hospital of the PLA, Beijing 100853; 2. Beijing Four Rings Bio-Pharmaceutical Co. Ltd., Beijing 100176; China) |
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| Abstract: | Objective： Using methylene blue spectrophotometric method to determine the content of SDS in recombinant biological products. Methods： The samples should be diluted in proportion, then mixed with methylene blue, added with chloroform for extraction, finally, using ultraviolet spectrophotometer to determine the OD value of the samples. Results： When the SDS concentration was in the range of 0-0.01 mg/mL, SDS concentration and D651nm value showed a linear relationship. Conclusion： The method can determine the content of SDS in samples with a good precision. |
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| Keywords: | methylene blue spectrophotometric method SDS recombinant biological products |
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