Interleukin-10 regulates the inflammasome-driven augmentation of inflammatory arthritis and joint destruction |
| |
| Authors: | Claire J Greenhill Gareth W Jones Mari A Nowell Zarabeth Newton Ann K Harvey Abdul N Moideen Fraser L Collins Anja C Bloom Rebecca C Coll Avril AB Robertson Matthew A Cooper Marcela Rosas Philip R Taylor Luke A O’Neill Ian R Humphreys Anwen S Williams Simon A Jones |
| |
| Institution: | .Cardiff Institute of Infection and Immunity, The School of Medicine, Cardiff University, Heath Park Campus, Cardiff, CF14 4XN Wales UK ;.Trinity Biomedical Sciences Institute, School of Biochemistry and Immunology, Trinity College Dublin, 152-160 Pearse Street, Dublin 2, Ireland ;.Institute for Molecular Bioscience, The University of Queensland, St Lucia Campus, Brisbane 4072, QLD Australia |
| |
| Abstract: | IntroductionActivation of the inflammasome has been implicated in the pathology of various autoinflammatory and autoimmune diseases. While the NLRP3 inflammasome has been linked to arthritis progression, little is known about its synovial regulation or contribution to joint histopathology. Regulators of inflammation activation, such as interleukin (IL)-10, may have the potential to limit the inflammasome-driven arthritic disease course and associated structural damage. Hence, we used IL-10-deficient (IL-10KO) mice to assess NLRP3 inflammasome-driven arthritic pathology.MethodsAntigen-induced arthritis (AIA) was established in IL-10KO mice and wild-type controls. Using histological and radiographic approaches together with quantitative real-time PCR of synovial mRNA studies, we explored the regulation of inflammasome components. These were combined with selective blocking agents and ex vivo investigative studies in osteoclast differentiation assays.ResultsIn AIA, IL-10KO mice display severe disease with increased histological and radiographic joint scores. Here, focal bone erosions were associated with increased tartrate-resistant acid phosphatase (TRAP)-positive cells and a localized expression of IL-1β. When compared to controls, IL-10KO synovium showed increased expression of Il1b, Il33 and NLRP3 inflammasome components. Synovial Nlrp3 and Casp1 expression further correlated with Acp5 (encoding TRAP), while neutralization of IL-10 receptor signaling in control mice caused increased expression of Nlrp3 and Casp1. In ex vivo osteoclast differentiation assays, addition of exogenous IL-10 or selective blockade of the NLRP3 inflammasome inhibited osteoclastogenesis.ConclusionsThese data provide a link between IL-10, synovial regulation of the NLRP3 inflammasome and the degree of bone erosions observed in inflammatory arthritis.Electronic supplementary materialThe online version of this article (doi:10.1186/s13075-014-0419-y) contains supplementary material, which is available to authorized users. |
| |
| Keywords: | |
|
|
