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杯萼海桑和格氏海桑自然杂交的分子证据
引用本文:裘索,周仁超,黎运钦,Sonjai HAVANOND,Chanop JAENGJAI,施苏华.杯萼海桑和格氏海桑自然杂交的分子证据[J].植物分类学报,2008,46(3):391-395.
作者姓名:裘索  周仁超  黎运钦  Sonjai HAVANOND  Chanop JAENGJAI  施苏华
作者单位:Suo QIU(State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat-Sen University, Guangzhou 510275, China)  Ren-Chao ZHOU(State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat-Sen University, Guangzhou 510275, China)  Yun-Qin LI(Experimental Center of Fundamental Teaching, Zhuhai Campus, Sun Yat-Sen University, Zhuhai 519802, China)  Sonjai HAVANOND(Department of Marine and Coastal Resources, Phayathai, Bangkok 10400, Thailand)  Chanop JAENGJAI(Department of Marine and Coastal Resources, Phayathai, Bangkok 10400, Thailand)  Su-Hua SHI(State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat-Sen University, Guangzhou 510275, China)
基金项目:国家重点基础研究发展计划(973计划) , 国家自然科学基金 , The Chang Hung-Ta Science Foundation of Sun Yat-sen University and the Young Teacher Foundation of Sun Yat-Sen University
摘    要:

关 键 词:chloroplast  DNA  mangroves  natural  hybridization  nuclear  gene  Sonneratia.  海桑  自然杂交  分子证据  alba  Sonneratia  hybridization  natural  evidence  event  Molecular  data  existed  nucleotide  regions  Sequencing  results  sequences  matching  acquired  determine
修稿时间:2008年2月20日

Molecular evidence for natural hybridization between Sonneratia alba and S. griffithii
Suo QIU,Ren-Chao ZHOU,Yun-Qin LI,Sonjai HAVANOND,Chanop JAENGJAI,Su-Hua SHI.Molecular evidence for natural hybridization between Sonneratia alba and S. griffithii[J].Acta Phytotaxonomica Sinica,2008,46(3):391-395.
Authors:Suo QIU  Ren-Chao ZHOU  Yun-Qin LI  Sonjai HAVANOND  Chanop JAENGJAI  Su-Hua SHI
Institution:1. State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, Sun Yat-Sen University, Guangzhou 510275, China
2. Experimental Center of Fundamental Teaching, Zhuhai Campus, Sun Yat-Sen University, Zhuhai 519802, China
3. Department of Marine and Coastal Resources, Phayathai, Bangkok 10400, Thailand
Abstract:Interspecific hybridization has been frequently observed in the mangrove genus Sonneratia. However, no natural hybridization has been reported between Sonneratia alba and S. griffithii to date, despite their overlapping distribution in the coast of Andaman Sea. In this study, cysteine proteinase inhibitor gene (cpi) from the nuclear genome, and two intergenic spacers (trnL-trnF and trnV-trnM) from the chloroplast genome, were sequenced to determine whether natural hybridization took place between the two species. Our results revealed two distinct types of cpi sequences from the putative hybrid matching those acquired from S. griffithii and S. alba, respectively. Sequencing of the chloroplast trnL-trnF and trnV-trnM regions showed that S. alba differed from S. griffithii by one nucleotide in each region, and the putative hybrid had the identical sequences with S. griffithii. Molecular data demonstrated clearly that there indeed existed natural hybridization between S. alba and S. griffithii, and that S. griffithii was the maternal parent in this hybridization event.
Keywords:chloroplast DNA  mangroves  natural hybridization  nuclear gene  Sonneratia  
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