利用RAPD分子标记评价仲彬草属的种间关系

利用随机扩增多态性DNA(RAPD)技术分析了14种仲彬草属Kengyilia植物的种间关系。对34个OPRON公司十聚体随机引物进行多态性筛选，20个(58．8％)能产生多态性。14个引物产生的112个DNA片断，用于计算种间Jaccard遗传相似性系数分析，在NTSYS程序中利用UPGMA构建系统发育树状图。分析结果表明：(1)14个Kengyilia物种存在较大的遗传多样性；(2)青藏高原的物种与新疆的物种 的RAPD变异极大；(3)形态相似、地理分布一致的物种有一定的亲缘关系，聚类在一起；(4)RAPD结果与形态学和细胞学等分析结果一致。RAPD分析方法将为Kengyilia系统分类提供DNA水平上丰富的资料。

Relationships among Kengyilia species assessed by RAPD markers

WT5BZ]To assess the interspecific relationships in Kengyilia, fourteen species of Kengyilia were used for RAPD assay. Of the 34 random primers tested, twenty (58.8%) produced polymorphic products. A total of 112 products amplified from 14 primers were selected for RAPD analysis. Eighty three (74.1%) amplified products were found to be polymorphic among 14 species. Data were used to generate Jaccard's similarity coefficients and to construct a dendrogram using UPGMA in the NTSYS computer programs. It is concluded from this study that: (1) There was great genetic diversity among the 14 species of Kengyilia; (2) Distinct RAPD variations existed between the species from the Qinghai Xizang Plateau and those from Xinjiang; (3) The species with similar morphological characters and the species from the same areas or neighboring geographical regions were clustered together; (4) RAPD results are comparable with those obtained from studies on morphology and cytology. RAPD assay is a useful additional method for assessing the relationships among the species in Kengyilia.