芍药属牡丹组的系统学研究——基于RAPD分析

芍药属牡丹组(Paeonia sect．Moutan DC)是落叶亚灌木，其野生类群为我国特有。长期以来不同 学者根据形态性状对这个组中种的分类处理不断修正，不断有新种描述。我们采用RAPD标记分析了 牡丹组种内与种间遗传关系。从10个RAPD引物获得121个多态位点。用UPGMA方法构建的树系 图表明每个种的所有个体都各自聚为一支，种内的相似性系数为0.60～0.90，因此现有的7个种能很好 地区分开来。P．delavayi与P．ludlowii相似性系数为0.60，聚为一支；P．jishanensis与P．rockii、P. ostii、P．qiui以及P．decomposita之间的相似性系数为0.48，聚为一大支。这两支与肉质花盘亚组和革 质花盘亚组相对应。这些结果与洪德元根据形态性状对该组所做的分类处理基本相符。我们认为RAPD技术用于牡丹基因组分析是灵敏而行之有效的工具。

Systematic studies on Paeonia sect. Moutan DC. based on RAPD analysis

Plants in Paeonia sect.Moutan DC.,whose wild types are endemic to China, are deciduous subshrubs.Taxonomic treatments of most species in this section have long been in dispute. To address this question, both intraspecific and interspecific relationships of the species in this section were analyzed using RAPD markers. The dendrogram constructed by UPGMA showed that the accessions of the same species were always grouped together earlier than those of different species.The intraspecific similarity coefficients ranged from 0.60 to 0.90 , grouping precisely those species of the same subsection together. Hence,the seven species under question can be well distinguished from each other. The similarity coefficient between P.delavayi and P.ludlowii was 0.60, and they were clustered in a clade. The similarity coefficients between P. jishanensis and the three species P.rockii, P.ostii, P.qiui, and between P.jishanensis and P.decomposita were both 0.48. These five species were clustered in another clade. These two clades corresponded well to Subsect. Delavayanae and Subsect. Vaginatae. Our results support the taxonomic treatment of Sect. Moutan recently proposed by Hong (1998,1997).