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Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology
Authors:Gerard Muyzer  Kornelia Smalla
Institution:(1) Max-Planck-Institute for Marine Microbiology, Celsiusstraße 1, D-28359 Bremen, Germany;(2) Biologische Bundesanstalt für Land- und Forstwirtschaft, Messeweg 11/12, D-38104 Braunschweig, Germany
Abstract:Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented. Furthermore, the potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so important.
Keywords:DGGE  genetic fingerprinting  microbial ecology  molecular microbial ecology  PCR  rRNA  TGGE
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