Silencing of the type 1 insulin-like growth factor receptor increases the sensitivity to apoptosis and inhibits invasion in human lung adenocarcinoma A549 cells |
| |
Authors: | Zhiyuan Ma Aiqiang Dong Minjian Kong Jianfang Qian |
| |
Institution: | (1) Department of Cardiothoracic Surgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China;(2) Department of Thoracic and Cardiovascular Surgery, Shanghai Jiao Tong University Affiliated First People’s Hospital, Shanghai, 200080, China |
| |
Abstract: | The type 1 insulin-like growth factor receptor (IGF-1R), which is over-expressed or activated in many human cancers, including
lung cancer, mediates cancer cell proliferation and metastasis. Several studies indicate that blocking IGF-1R expression can
inhibit tumor cell proliferation and metastasis. In this study, inhibition of the endogenous IGF-1R by recombinant adenoviruses
encoding short hairpin RNAs against IGF-1R was found to significantly suppress IGF-1R expression, arrest the cell cycle, enhance
the apoptotic response, and inhibit proliferation, adhesion, invasion and migration in A549 cells. Moreover, silencing IGF-1R
decreases the expression of invasive-related genes including matrix metalloproteinase-2 (MMP-2), MMP-9, and urokinase-plasminogen
activator (u-PA), and the phosphorylation of Akt and ERK1/2. These results suggest that the silencing of IGF-1R has the potential
to be an effective cancer gene therapy strategy for human lung cancer. |
| |
Keywords: | IGF-1R Lung cancer Short hairpin RNA MMPs Apoptosis Metastasis |
本文献已被 PubMed SpringerLink 等数据库收录! |
|